P27、SKP2在宫颈癌组织中的表达及相关性分析

Expression and relationship of P27 and SKP2 protein in cervical carcinoma

目的研究蛋白P27和SKP2在宫颈癌组织中的表达及与临床各项病理指标的关系。方法选取大庆油田总医院病理科2004年6月至2006年6月40例宫颈癌、10例正常宫颈组织石蜡切块。用免疫组化(SP法)检测P27和SKP2的表达情况,并与临床病理特征进行比较。结果 P27和SKP2蛋白阳性表达均定位于癌细胞的细胞核内;P27和SKP2在宫颈癌组织中的阳性表达率分别为32.5%(13/40)和70.0%(28/40),在正常宫颈组织中的阳性表达率分别为90.0%(9/10)和20.0%(2/10),两者在宫颈癌和正常宫颈组织中的表达差别有显著意义(P<0.01);P27和SKP2在宫颈癌的表达与分化程度、临床分期、有无淋巴结转移有关(P<0.05),与病理类型无关(P>0.05);通过Spearm an等级相关分析结果显示,P27和SKP2在宫颈癌组织中的表达两者之间呈显著负相关(R=-0.433,P=0.005)。结论 P27和SKP2蛋白表达的情况与宫颈癌的发生和转移有密切关系,检测P27和SKP2的表达可作为了解宫颈癌生物学行为的参考指标。

Objective：To investigate the expression of targeted protein P27 and SKP2 in cervical carcinoma and their relationship with clinicpathologic characters,and the correlation between expression of SKP2 and P27 in cervical carcinoma.Methods：We selected 40 cases of human cervical carcinoma tissues,and 10 normal cervical tissues in the first hospital of daqing oil field from June 2004 to June 2006,using immunohistochemistry（two steps methods） to detect the expression of P27 an SKP2 and comparing their pathologic features.Results：The positive expression of P27 and SKP2 protein was mainly in cellular nucleus;the positive rate of P27 and SKP2 expression in cervical carcinoma were 32.5%（13/40） and 70.0%（28/40）,while in normal cervical tissues were 90.0%（9/10） and 20.0%（2/10）.They were both have significantly differences in cervical carcinoma and normal cervical tissues（P0.01）;The expressions of P27 and SKP2 were correlated with clinical stage,histological differentiation,and lymph node metastasis（P0.05）,but not with the carcinoma types（P0.05）;through spearman the results of the analysis indicated that P27 and SKP2 expression in cervical carcinoma has negative correlation（R=-0.433,P=0.005）.Conclusion：The results show that the expression of biologic behavior of cervical carcinoma.