Fas/FasL及Bcl-2在子痫前期胎盘的表达及意义

Expressions and significances of Fas/FasL and Bcl-2 in placentae of cases with preeclampsia

目的:探讨Fas抗原(Fas)及其配体(FasL)、Bcl-2在子痫前期患者胎盘合体滋养细胞中的表达变化及其意义。方法:采用免疫组化链霉菌抗生物素蛋白-过氧化物酶(SP)连接法检测20例正常晚期妊娠妇女(正常晚孕组)、20例轻度子痫前期产妇(轻度子痫前期组)及20例重度子痫前期产妇(重度子痫前期组)胎盘组织中Fas、FasL及Bcl-2表达水平。结果:Fas、FasL及Bcl-2主要表达于胎盘合体滋养细胞胞质及胞膜中。FasL、Bcl-2在轻度子痫前期组(62.28±4.92、80.67±6.19)及重度子痫前期组(41.74±6.38、64.42±5.43)胎盘中的表达均低于正常晚孕组(80.72±6.01、92.49±7.88),分别与正常晚孕组比较,差异均有统计学意义(P<0.01)。Fas在轻度子痫前期组(55.94±4.35)及重度子痫前期组(75.98±6.01)胎盘中的表达均高于正常晚孕组(40.58±5.43),分别与正常晚孕组比较,差异均有统计学意义(P<0.01)。结论:Fas、FasL及Bcl-2在子痫前期胎盘合体滋养细胞中表达失衡,可能与子痫前期的发病及发展有关。

Objective：To explore the changes of expressions of Fas antigen（Fas） and its ligand（FasL）,Bcl-2 in syncytiotrophoblast of placentae of pregnant women with preeclampsia and their significances. Methods：Immunohistochemical SP method was used to detect the expression levels of Fas,FasL and Bcl-2 in placental tissues of 20 normal late pregnant women（normal late pregnancy group）,20 pregnant women with mild preeclampsia（mild preeclampsia group） and 20 pregnant women with severe preeclampsia（severe preeclampsia group）. Results：Fas,FasL and Bcl-2 mainly expressed in cytoplasm and cell membrane of placental syncytiotrophoblast.The expression levels of FasL and Bcl-2 in mild preeclampsia group and severe preeclampsia group were（62.28±4.92）,（80.67±6.19） and（41.74±6.38）,（64.42±5.43）,respectively,which were significantly lower than those in normal late pregnancy group（（80.72±6.01）,（92.49±7.88））（P0.01）.The expression levels of Fas in mild preeclampsia group and severe preeclampsia group were（55.94±4.35） and（75.98±6.01）,respectively,which were significantly higher than that in normal late pregnancy group（40.58±5.43）（P0.01）. Conclusion：The unbalance of Fas,FasL and Bcl-2 expressions in placental syncytiotrophoblast of pregnant women with preeclampsia may be related to the occurrence and development of preeclampsia.