转化生长因子β1T869C基因多态性与子宫内膜异位症

Polymorphism of transforming growth factor β1 T869C gene and endometriosis

目的:探讨转化生长因子TGFβ1T869C基因多态性及其血清水平与子宫内膜异位症的关系。方法:用聚合酶链反应扩增TGFβ1T869C基因片段,PstⅠ内切酶进行限制性酶切反应并经测序证实。结果:子宫内膜异位症组TGFβ1 T869 C/C、C/T、T/T表型频率分别为0.210 0、0.600 0、0.190 0,对照组分别为0.250 0、0.580 0、0.170 0;子宫内膜异位症组TGFβ1 T869C、TGFβ1 T869T基因频率分别为0.510 0、0.490 0。对照组分别为0.540 0、0.460 0,TGFβ1T869C各种基因型频率在内异症组与对照组之间的差异无统计学意义(P>0.05)。结论:TGFβ1T869C基因多态性与子宫内膜异位症无关联。

Objective：To explore the relationship between tumor necrosis factor（TGF） β1T869C gene polymorphism,its serum level and endometriosis. Methods：PCR was used to amplify the fragments of TGF β1T869C gene,RELP was performed by Pst Ⅰ endonuclease,then the results were confirmed by gene sequencing. Results：The frequencies of TGF β1 T869 C/C,C/T,T/T phenotypes in endometriosis group were 0.210 0,0.600 0 and 0.190 0,respectively,while in control group,the frequencies were 0.250 0,0.580 0 and 0.170 0,respectively;the gene frequencies of TGFβ1 T869C and TGFβ1 T869T in endometriosis group were 0.510 0 and 0.490 0,respectively,while in control group,the frequencies were 0.540 0 and 0.460 0,respectively;there was no significant difference in the frequencies of various TGF β1T869C genotypes between endometriosis group and control group（P0.05）. Conclusion：There is no correlation between TGF β1T869C gene polymorphism and endometriosis.