摘要
目的建立HPLC法测定北合欢中槲皮素和山奈素的含量。方法采用Diamonsil C18色谱柱;甲醇-0.4%磷酸溶液（50∶50）为流动相;检测波长为360nm;柱温35℃。结果槲皮素在0.0088~0.5262μg范围内呈良好的线性关系,r=0.9997;平均回收率（n=6）为98.8%,RSD=3.0%。山奈素在0.0242~1.4496μg范围内呈良好的线性关系,r=0.9999;平均回收率（n=6）为100.5%,RSD=2.0%。结论本法简便,准确,重现性好,可作评价北合欢的质控方法。
Objective To establish a method for the determination of quercetin and kaempferide in Celatrus orbiculatus Thunb.Methods A column of Diamonsil C18 was adopted,and the mobile phase was the mixture of methanol-0.4%phosphoric acid（50∶50）at a flow rate of 1.0mL·min-1.The wavelength was set at 360nm.Results There was a good linear relationship of quercetin in the concentration range of 0.0088～0.5262μg（r=0.9997）.The average recoverise（n=6） were 98.8%（RSD=3.0%）.There was a good linear relationship of kaempferide in the concentration range of 0.0242～1.4496μg（r=0.9999）.The average recoverise（n=6） were 100.5%（RSD=2.0%）.Conclusion The method is simple andaccurate.It can be used for the quantitative analysis of Celatrus orbiculatus Thunb.
出处
《中国药事》
CAS
2011年第7期647-649,共3页
Chinese Pharmaceutical Affairs
基金
吉林省科技厅发展计划重点资助项目(编号20070902-01)
关键词
北合欢
槲皮素
山奈素
HPLC
Celatrus orbiculatus Thunb
quercetin
kaempferide
determination
HPLC
