近红外光谱法快速测定卡托普利片的含量

Rapid Determination of Captopril Tablets by NIR Spectroscopic Method

目的采用近红外漫反射光谱技术快速测定卡托普利片剂的含量。方法以全国36个企业生产的209批卡托普利片作为分析对象,分别采集了3次近红外漫反射光谱,并求得平均光谱,建立定量分析模型。光谱预处理方法为一阶导数和矢量归一化;平滑点：17;谱段范围：11220.5-7344.1、6572.6-5793.5cm-1;回归方法为偏最小二乘法（PLS）。结果 179个样品经内部交叉验证建立预测模型,浓度范围为8.79%~36.82%（mg.mg-1）,决定系数（R2）为98.93%,内部交叉验证预测均方根误差（RMSECV）为0.811。用30个样品进行外部验证,决定系数（R2）为98.56%,外部验证预测均方根误差（RMSEP）为0.818,平均绝对偏差为0.55%（mg.mg-1）,平均相对偏差为2.81%。结论该方法快速简便、准确有效,可以满足药品现场快速检查的需要。

Objective Determine captopril in Captopril Tablets rapidly by NIR（near-infrared） spectroscopy.Methods Measure NIR spectra of 209 batches Captopril Tablets from 36 different manufacturers 3 times each,then develop the model using the average spectra.The quantitative model was established by partial least squares（PLS） algorithm with 1st derivative and vector normalization as the preprocessing method and 11220.5~7344.1,6572.6~5793.5cm-1 as the spectral ranges.Results 179 batches of Captopril Tablets samples,whose concentration are between 8.79% and 36.82%（mg·mg-1） were selected as calibration set.The rootmean square errors of cross validation（RMSECV） was 0.811 and determination coefficients（R2） was 98.93%.The rootmean square error of prediction（RMSEP） of 30 batches samples in test was 0.818.The determination coefficients（R2） was 98.56%.The average absolute difference of prediction for test set was 0.55% and the mean relative difference of prediction for test set was 2.81%.Conclusion The NIR method can determine Captopril Tablets accurately and quickly,thus can meet the need of rapid drug testing on site.