氢气饱和生理盐水对氧中毒肺损伤的防护作用

Protective effect of saturated hydrogen saline on hyperoxic lung injury

目的 研究氢气饱和生理盐水对于氧中毒肺损伤的防护作用.方法雄性Sprague-Dawley大鼠（SD大鼠）48只,按数字表法随机均分为4组：常压空气对照组,生理盐水模型组,氢饱和生理盐水模型组和氮饱和生理盐水模型组.除对照组外,其他各组常压下持续吸100%医用纯氧60 h,建立慢性氧中毒损伤模型,并在吸氧过程中,分别用重盐水、氢气饱和生理盐水、氮气饱和生理盐水对动物进行处理.应用胸腔积液、肺含水量和HE染色来观察慢性氧中毒的损伤程度.检测肺组织中丙二醛（MDA）、超氧化物歧化酶（SOD）的表达水平以及DNA损伤指标8-羟基脱氧鸟嘌呤（8-OHDG）的变化.结果 与生理盐水模型组相比,氢气饱和生理盐水能更明显减轻慢性纯氧暴露导致的肺损伤,减少胸腔积液和肺水肿,氧化损伤指标明显减轻.然而氮气饱和生理盐水对于肺损伤没有保护作用.结论 高浓度氧暴露过程中同时应用氢气饱和生理盐水可以减轻氧中毒肺损伤.

Objective To investigate the protective effect of saturated hydrogen saline on acute lung injury induced by hyperoxia in rats. Methods Forty-eight male SD rats were randomly divided into 4 groups： the normal air control group, the physiological saline group, the saturated hydrogen saline group and the saturated nitrogen saline group. With the exception of the control group, the animals in the other groups exposed continuously to pure medical oxygen （02 concentration 〉 98% ） for 60 h to develop the chronic oxygen poisoning model. Then, the animals were treated with the physiological saline, the saturated hydrogen saline and the saturated nitrogen saline in the process of oxygen exposure. Severity of acute lung injury induced by hyperoxia （HALI） was assessed by the volume of pleural effusion, water content and Hematoxylin and Eosin （HE） staining. Malondialdehyde （MDA） and the expression of superoxidase dismutase （SOD） were monitored for assessment of oxidative stress, and changes in DNA oxidation product, 8-hydroxy-desoxyguanosine （ 8- OHDG） were also monitored. Results When a comparison was made with the physiological saline group, saturated hydrogen saline treatment could significantly relieve lung injury induced by chronic pure oxygen exposure as indicated by HE staining, decrease volume of pleural effusion and pulmonary edema. Moreover, saturated hydrogen saline treatment could inhibit lipid and DNA oxidation. Experiments showed that saturated nitrogen saline treatment had no protective effect on hyperoxic lung injury. Conclusions Saturated hydrogen saline treatment could alleviate lung injury induced by hyperoxia.