基于人工消化液与密闭消化器的酶法测定豆粕鸭代谢能值的研究

Study on the Metabolizable Energy of Soybean Meal in Duck Determined Using Enzymatical Method Based on Simulative Digestive Juice and Sealed Digestive Apparatus

本试验旨在通过探讨基于人工消化液与密闭消化器的酶法条件下酶法与排空强饲法的测试精度及测定豆粕鸭代谢能值的差异,为鸭饲料养分生物学效价酶学评定方法的设计提供参考。酶法与排空强饲法测试精度的比较研究采用完全随机设计,其中酶法每个消化阶段设5个重复,每个重复1个样品,排空强饲法设4个重复测定,每个重复3只试验鸭,比较两方法间重复测定的极差、相对标准差偏差。酶法与排空强饲法测定豆粕鸭代谢能值的比较研究中,采用配对设计,对25个豆粕样品的酶水解物能值与排空强饲代谢能作系统比较。结果表明,鸭排空强饲法重复测定的日粮干物质消化率极差为2.89%,相对偏差为1.57%,代谢能值极差为0.36MJ.kg-1,相对标准偏差为1.13%。酶法重复测定的日粮干物质消化率极差和相对标准偏差分别为1.57%和1.13%,其测试的精度可以达到鸭排空强饲法类似的测试精度。25个豆粕样品的鸭排空强饲法真代谢能(TME)平均值、酶水解物能值平均值分别为13.33和13.55MJ.kg-1 DM,两平均值间无显著性差异(P>0.05)。在考虑到允许误差的前提下,本试验所用酶法准确估测豆粕鸭代谢能值的概率为68%(n=25,差值为0.85MJ.kg-1以内)。

The comparison of analytical precision between enzymatical and empting-force feeding methods,and the difference of metabolizable energy of soybean meal in duck determined by using the two methods were investigated to study the feasibility of enzymatical method used for determination of nutrient digestibility in duck feedstuff.In the comparison experiment of analytical precision between enzymatical and empting-force feeding methods,a completely randomized design was adapted.There were five replicates with one simulative digestion per replicate at each digestion stage of enzymatical method,and four replicates with three ducks per replicate in empting-force feeding method.The range and relative standard deviation of these two method were calculated.In the comparison experiment of metabolizable energy of soybean meal determined using the two methods,the paried design was adapted to compare the difference of enzymatical hydrolyzed gross energy and metabolizable energy of 25 soybean meal.The results showed that the range and relative standard deviation of dietary dry matter digestibility and metabolizable energy determined using empting-force feeding method were 2.89% and 1.57%,0.36 MJ·kg-1 and 1.13%,respectively.The range and relative standard deviation of dietary dry matter digestibility measured using enzymatical method were 1.57% and 1.13%,which meant the precision of enzymatical method was similar to empting-force feeding method.The mean of true metabolizable energy and enzymatical hydrolyzed gross energy of 25 soybean meal were 13.33 and 13.55 MJ·kg-1 DM（P0.05）,respectively.Taking the permitted error into account,the probability for accurately predicting the metabolizable energy of soybean meal in duck was about 68% using enzymatic method.