非酒精性脂肪肝模型小鼠的建立

Establishment of non-alcoholic fatty liver model in mice

背景:以往非酒精性脂肪肝模型建立的常用诱导方法均有其局限性,因此,有必要建立高质量的非酒精性脂肪肝模型。目的:拟利用复合高脂饮食和低浓度四氯化碳诱导小鼠非酒精性脂肪肝模型。方法:取昆明小鼠随机分为3组:对照组,高脂模型组和高脂模型+四氯化碳组。观察饲养第2,4,6和8周末肝脏形态和病理变化,测定第8周末血清丙氨酸氨基转移酶,天冬氨酸氨基转移酶,胆固醇和三酰甘油,以及肝脏胆固醇和三酰甘油水平。结果与结论:复合高脂饮食+5%四氯化碳腹腔注射的方式造模,第2周末开始出现炎症细胞浸润,第4周末出现少量脂滴,第6周末出现脂肪病变,第8周末在脂肪病变的基础上,部分小鼠出现了肝纤维化,8周末小鼠肝指数、血清丙氨酸氨基转移酶,天冬氨酸氨基转移酶,胆固醇和三酰甘油,肝脏胆固醇和三酰甘油浓度明显升高(P<0.05或P<0.01)。通过8周复合高脂饮食和5%四氯化碳腹腔注射成功建立小鼠非酒精性脂肪肝模型。

BACKGROUND：Methods for inducing non-alcoholic fatty liver model（NAFLM） should be improved.OBJECTIVE：To establish NAFLM in mice by high fat feeding and carbon tetrachloride.METHODS：Kunming mice were randomly divided into three groups：control,high fat,and high fat plus carbon tetrachloride.Liver morphological and pathological changes were observed at 2,4,6 and 8 weeks.Serum levels of total cholesterol（TC）,triglyceride（TG）,alanine transaminase（ALT） and aspartate transaminase（AST） and hepatic TC,TG changes were determined.RESULTS AND CONCLUSION：In high fat plus carbon tetrachloride group,inflammatory cell infiltration appeared in the mice liver after 2 weeks.There were a small amount of lipid droplets at 4 weeks,and steatosis emerged after 6 weeks.Hepatic fibrosis began to appear at 8 weeks.The serum levels of total TC,TG,ALT and AST,and hepatic TC,TG were significantly advanced in high fat plus carbon tetrachloride group compared with control group at 8 weeks（P 0.05 or P 0.01）.The NAFLM can be established successfully by feeding with high fat food and injecting 5% carbon tetrachloride.