自体富含血小板血浆对兔骨折愈合的影响

Role of autologous platelet-rich plasma on fracture healing in rabbits

背景:研究表明富含血小板血浆常通过同源异体制备,可应用于促进扁骨和松质骨的骨折愈合。目的:用Landsbergs法制备自体富含血小板血浆,观察其对兔桡骨骨折愈合影响。方法:将新西兰兔随机分成富含血小板血浆组和对照组,分别建立桡骨中下1/3处骨折模型,富含血小板血浆组中加入制备的自体富含血小板血浆凝胶,对照组建立骨折模型后直接缝合。采用Landsbergs法制备自体富含血小板血浆,并植入富含血小板血浆组的骨折端,并行骨折断端石膏外固定。结果与结论:兔骨折标本在建模后1,2,4,6周均有不同程度骨痂形成。富含血小板血浆组在建模后第4周X射线进行影像学评分明显高于对照组(P<0.05)。免疫组织化学检测显示:富含血小板血浆组Ⅰ型胶原量明显的高于对照组(P<0.05),可持续4周左右。结果证实,富含血小板血浆可通过增加骨痂中骨岛量和加速Ⅰ型胶原合成而明显促进长骨骨折愈合。

BACKGROUND：Platelet-rich plasma（PRP） is often prepared with autalloploid and can be used to promote the fracture healing of flat bone and cancellous bone.OBJECTIVE：To prepare PRP with Landsberger＇s method and to analyzes the therapeutic role of PRP on the fracture healing of radius in rabbits.METHODS：New Zealand rabbits were randomly divided into a control group and a PRP treated group.Unilateral open femoral fracture models were established in the lower 1/3 radius bone.PRP group received was treated with autologous PRP gel,while control group was only sutured after fracture modeling.Autologous PRP was prepared by Landsberger＇s method and then implanted to the fracture site,followed by plaster immobilization of the fracture fragments.RESULTS AND CONCLUSION：The fracture specimens exhibited varying degrees of callus formation at 1,2,4,6 weeks.X-ray score in the PRP group was significantly higher than that in control group at 4 weeks（P 0.05）.Immunohistochemistry showed that,type I collagen content in the PRP treatment group was significantly higher than that in the control groups for 4 weeks（P 0.05）.PRP accelerates bone fracture healing of rat femurs by increasing expression of type I collagen and the number of bone island.