期刊文献+

灯盏花素对腹膜透析液诱导人腹膜间皮细胞转化生长因子β1的影响 被引量:5

Effect of breviscapine on transforming growth factor beta 1 in human peritoneal mesothelial cells induced by peritoneal dialysis solution
下载PDF
导出
摘要 背景:从不同层面了解灯盏花素阻止/延缓腹膜功能衰竭的作用及其机制,从而在临床上推广使用灯盏花素来阻止/延缓腹膜功能衰竭从而延长终末期肾脏病患者腹膜透析时间、提高透析质量、减少透析失败率,提高腹膜透析远期疗效具有广泛的应用前景。目的:观察灯盏花素对腹膜透析液诱导的人腹膜间皮细胞转化生长因子β1分泌及其增殖活性的影响。方法:体外培养人腹膜间皮细胞,分为5组:分别为对照组、腹膜透析液组、灯盏花素终浓度为5,10,20μmol/L组。检测各组上清液中转化生长因子β1的水平以及间皮细胞的增殖活性。结果与结论:腹膜间皮细胞在腹膜透析液诱导下,转化生长因子β1分泌显著增加、细胞增殖活性显著降低。灯盏花素5μmol/L组转化生长因子β1分泌低于腹膜透析液组(P<0.05),细胞增殖活性高于腹膜透析液组(P<0.05);灯盏花素10,20μmol/L组转化生长因子β1分泌显著低于腹膜透析液组(P<0.01),细胞增殖活性显著高于腹膜透析液组(P<0.01)。结果显示灯盏花素可以抑制腹膜间皮细胞转化生长因子β1分泌,拮抗腹膜透析液对腹膜间皮细胞增殖活性的抑制作用。 BACKGROUND:Based on the understanding of breviscapine effects and mechanism on inhibiting and delaying peritoneal failure,breviscapine is expected to be widely introduced to prolong peritoneal dialysis time,improve the quality of dialysis,and reduce dialysis failure rate so as to promote long-term effect of peritoneal dialysis in patients with end stage renal diseases.OBJECTIVE:To observe the effects of breviscapine on the secretion of transforming growth factor beta 1(TGF-β1) and proliferation activity of human peritoneal mesothelial cells(HPMCs) cultured in vitro with peritoneal dialysis solution(PDS).METHODS:HPMCs were cultured in vitro and divided into five groups:control group,PDS group,5 μmol/L breviscapine group,10 μmol/L breviscapine group,and 20 μmol/L breviscapine group.The levels of TGF-β1 and proliferation of HPMCs in supernatant were detected and compared.RESULTS AND CONCLUSION:Significantly increased levels of TGF-β1 and decreased cell proliferation activity were observed in the PDS group.The level of TGF-β1 in the PDS group was higher than that in the 5 μmol/L breviscapine group(P 0.05),and significantly higher than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group(P 0.01).The proliferation activity in the PDS group was lower than that in the 5 μmol/L breviscapine group(P 0.05),and significantly lower than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group(P 0.01).The results indicated that breviscapine can reduce the secretion of TGF-β1 in cultured HPMCs.Also it can restore the reduced cell proliferation activity caused by PDS.
出处 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2011年第24期4437-4439,共3页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 贵州省卫生厅优秀医学青年人才基金(No.gzwkj2009-2-009)~~
  • 相关文献

参考文献15

  • 1杜钢军,张硕,林海红,王梅,姬利延.灯盏花素对博来霉素诱导小鼠肺纤维化的保护作用[J].中国药理学通报,2009,25(2):160-163. 被引量:25
  • 2Stylianou E, Jenner LA, Davies M, et al. Isolation, culture and characterization of human peritoneal mesothelial cells.Kidney Int. 1990;37(6): 1563-1570.
  • 3Aguilera A, Yanez-Mo M, Selgas R, et al. Epithelial to mesenchymal transition as a triggering factor of peritoneal membrane fibrosis and angiogenesis in peritoneal dialysis patients. Curr Opin Investig Drugs. 2005;6(3):262-268.
  • 4Smit W, Parikova A, Struijk DG, et al. The difference in causes of early and late ultrafiltration failure in peritoneal dialysis.Perit Dial Int. 2005;25 Suppl 3:S41-45.
  • 5周伟东,魏连波,龙海波,张志锋.灯盏花素注射液对腹膜透析患者腹膜转运效能的影响[J].广东医学,2008,29(9):1563-1564. 被引量:4
  • 6Medcalf JF, Walls J, Pawluczyk IZ,et al. Effects of glucose dialysate on extracellular matrix production by human peritoneal mesothelial cells (HPMC): the role of TGF-beta.Nephrol Dial Transplant. 2001 ; 16(9): 1885-1892.
  • 7Zweers MM, Struijk DG, Smit W,et al. Vascular endothelial growth factor in peritoneal dialysis: a longitudinal follow-up.J Lab Clin Med. 2001 ;137(2): 125-132.
  • 8Beavis M J, Williams JD, Hoppe J,et al. Human peritoneal fibroblast proliferation in 3-dimensional culture: modulation by cytokines, growth factors and peritoneal dialysis effluent. Kidney Int. 1997;51 (1):205-215.
  • 9Naiki Y, Maeda Y, Matsuo K,et al. Involvement of TGF-beta signal for peritoneal sclerosing in continuous ambulatory peritoneal dialysis. J Nephrol. 2003; 16(1 ):95-102.
  • 10何蔚,曾繁典.灯盏花素对异丙肾上腺素引起大鼠心肌肥厚和纤维化的保护作用[J].中国药理学通报,2005,21(12):1514-1517. 被引量:25

二级参考文献77

共引文献94

同被引文献79

  • 1李乾,张桂英,孟燕妮.氯沙坦抗鼠肝纤维化的实验研究(英文)[J].中国现代医学杂志,2007,17(4):385-388. 被引量:9
  • 2Shimizu M, Ishibashi Y, Taki F, et al. Endothelin (B) receptor bloc1ker inhibits high glucose-induced synthesis of fibronectin in human peritoneal mesothelial ceils [ J ]. Petit Dial Int,2006,26 ( 3 ) : 393.
  • 3Aguilera A, Yez-Mo M, Selgas R, et al. Epithelial to mesenchymal transition as a triggering factor of peritoneal membrane fibrosis and angiogenesis in peritoneal dialysis patients [ J ]. Curt Opin Investig Drugs ,2005,6( 3 ) :262 - 268.
  • 4Margetts PJ, Oh KH, Kolb M. Transfoming growth factor beta: impor- tance in long-term peritoneal membrane changes [ J ]. Petit Dial Int ,2005,25 ( Suppl 3 ) : S15 - 17.
  • 5Schrijvers BF, Devriese AS, Flyvbjerg A. From hyperglycemia to diabetic kidney disease : the role of metabolic, hemodynamic, intra- cellular factor and growth factor/cytokines [ J ]. Endocr Rev, 2004, 25(6) :971 - 1010.
  • 6ThodisE,PassadakisP,VargemezisV. Peritonealdialysis:betterthan,equal to,or worse than hemodialysis Data worth knowing before choosing a dialysismodality[J].PeritDial Int,2001,(1):25-35.
  • 7Mendelssohn DC. Peritoneal dialysis and the future:the role of peritoneal dialysis in the overall management of end-stage renal disease[J].{H}Blood Purification,2003.20-28.
  • 8Vargha R,Endemann M,Kratochwill K. Ex vivo reversal of in vivo transdifferentiation in mesothelial cells grown from peritoneal dialysate efffluents[J].{H}Nephrology Dialysis Transplantation,2006.2943.
  • 9Aroeira LS,Aguilera A,Sánchez-Tomero JA. Epithe-lial to Mesenchymal Transition and Peritoneal Membrane Failure in Peritoneal Dialysis Patients:Pathologic Significance and Potential Therapeutic Interventions[J].{H}Journal of the American Society of Nephrology,2007.2004.
  • 10Loureiro J,Aguilera A,Selgas R. Blocking TGF-[β1 protects the peritoneal membrane from dialysate-induced damage[J].{H}Journal of the American Society of Nephrology,2011,(9):1682-1695.

引证文献5

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部