单侧输尿管梗阻模型大鼠肾间质纤维化过程中血小板衍生生长因子D的表达

Expression of platelet-derived growth factor-D in pathogenesis of unilateral ureteral obstruction during renal interstitial fibrosis

背景:血小板衍生生长因子在肾间质中通过诱导肾小管间质细胞增生、表型转化、炎性细胞浸润等导致肾小管间质纤维化。目的:观察血小板衍生生长因子D在单侧输尿管梗阻模型大鼠肾脏组织中的表达水平及随时间的演变情况。方法:将成年健康雄性SD大鼠60只随机分为模型组及假手术组,将模型组大鼠左侧输尿管结扎剪断建立单侧输尿管梗阻模型,假手术组大鼠不结扎剪断仅游离左侧输尿管。术后3,7,14,21,28d,通过免疫组化检测血小板衍生生长因子D在肾脏组织中的表达分布情况,实时荧光定量RT-PCR方法检测血小板衍生生长因子D mRNA的表达水平及变化。结果与结论:假手术组血小板衍生生长因子D仅少量表达于肾小球系膜细胞及血管平滑肌细胞,而在模型组,血小板衍生生长因子D同时表达于肾间质纤维化区域,随纤维化程度加重,表达增多。同时模型组血小板衍生生长因子D mRNA表达量较假手术组显著增多(P<0.05),且表达随时间延长逐渐增多。提示血小板衍生生长因子D在单侧输尿管梗阻模型肾间质纤维化过程中发挥着促纤维化的重要意义。

BACKGROUND：The platelet-derived growth factors（PDGF） are highly expressed in the diseased renal,and induce renal interstitial fibrosis through tubulointerstitial cells proliferation,phenotypic modulation and inflammatory cell infiltration.OBJECTIVE：To observe expression of the PDGF-D in kidney tissues in the process of renal interstitial fibrosis.METHODS：Totally 60 SD rats were randomly divided into the model and sham-surgery groups.In the model group,rats were constructed unilateral ureteral obstruction models by left ureteral ligation.Animals were sacrificed at 3,7,14,21 and 28 days after operation,and the expression and distribution of the PDGF-D in the kidney tissues were detected by immunohistochemistry,and the expression levels of PDGF-D mRNA in tissue samples were determined by real-time RT-PCR.RESULTS AND CONCLUSION：There were few PDGF-D expressed in glomerular mesangial cells and vascular smooth muscle cells in the sham-surgery group,but highly expressed in the model group,which were focally observed in interstitial cells in areas of tubulointerstitial fibrosis,and increased with time prolonged.The mRNA level of PDGF-D in the model group was obviously greater than that of the sham-surgery group（P 0.05）.These observations suggest that PDGF-D plays an important role in the pathogenesis of unilateral ureteral obstruction during renal interstitial fibrosis.