天然与重组水蛭素对大鼠随意皮瓣淤血模型超氧化物歧化酶、丙二醛、内皮素变化的影响

Effect of natural and recombinant hirudin on superoxide dismutase,malonaldehyde and endothelin in rat random skin flap congestion models

背景:一般认为天然水蛭素能够提高淤血皮瓣的成活率与其抗血栓、抗凝血酶有关,但其抗炎、抗氧化作用的具体机制尚不清晰。重组水蛭素效果与天然水蛭素的差异也未有深入研究。目的:探索天然、重组水蛭素对大鼠随意皮瓣淤血模型存活保护机制。方法:Wistar大鼠30只,背部设计随意皮瓣10cm×3cm制成淤血模型。皮瓣随机分为天然水蛭素组、重组水蛭素组和对照组,均于术后即刻、第3,5天注射在距皮瓣末端1.5cm和3.0cm处分别注射5U天然水蛭素、5U重组水蛭素和相同剂量生理盐水。观察术后第7天的皮瓣成活率、皮瓣组织细胞形态学改变以及超氧化物歧化酶、丙二醛、内皮素水平。结果与结论:与对照组相比,天然、重组水蛭素组皮瓣成活率高,皮瓣超氧化物歧化酶水平明显增多,内皮素、丙二醛水平减少。且天然水蛭素效果优于重组水蛭素。说明水蛭素能够提高随意皮瓣的成活率、超氧化物歧化酶的含量;减低皮瓣的坏死率、丙二醛、内皮素的含量。

BACKGROUND：Most scholars believed that the effect of hirudin can improve the survival rate of congestion flap based on its antithromotic and antithrombin activity.However,whether it has anti-inflammatory and antioxidant effects remains unclear.The disparity between recombinant hirudin and recombinant hirudin needs to be explored.OBJECTIVE：To investigate the protective effect of natural hirudin and recombinant hirudin on random skin flap survival in rats vein congestion models.METHODS：Thirty Wistar rats were employed to establish animal model of vein congestion.Dorsal random skin flap was prepared on each animal,10 cm×3 cm in size.The flaps were divided randomly into 3 groups.5 U natural hirudin,5 U recombinant hirudin or physiological saline was injected at 1.5 cm and 3.0 cm at distal part of flap immediately,3 and 5 days after operation.The 7 days flap survival,morphological changes of flap and superoxide dismutase（SOD）,endothelin（ET） and malonaldehyde（MDA） levels were measured RESULTS AND CONCLUSION：The flap survival of the natural and recombinant hirudin groups were greater than that of the control group,with increased SOD level and decreased ET and MDA levels.In particular,the effect was more manifest in the natural hirudin group.The findings demonstrated that hirudin can improve the flap survival rate and SOD content but reduce the rate of flap necrosis,MDA,and ET levels.