温敏性壳聚糖水凝胶对大鼠成骨细胞的毒性

Cytotoxicity of chitosan thermosensitive hydrogel to rat osteoblasts

背景：温敏性壳聚糖与多种细胞相容性良好,是组织工程中不可多得的优良载体,但其对成骨细胞毒性研究相对缺乏。目的：验证温敏性壳聚糖水凝胶对成骨细胞的毒性。方法：成骨细胞在温敏性壳聚糖水凝胶中进行培养,显微镜下观察细胞形态及扩增情况,同时,SD大鼠成骨细胞在不同浓度的温敏性壳聚糖水凝胶浸提液中体外培养24,48,72,96h,MTT法测定细胞相对增殖率,判断细胞毒性的级别。结果与结论：SD大鼠成骨细胞在温敏性壳聚糖水凝胶中培养24h内镜下观察呈圆形,48h后开始伸出触角并扩增;温敏性壳聚糖水凝胶浸提液中培养的各组细胞在不同时间点相对增殖率在92%~112%之间,各浓度的温敏性壳聚糖水凝胶材料浸提液的细胞毒性均为0级或1级,完全符合生物材料的安全评价标准。

BACKGROUND：Thermosensitive chitosan shows good compatibility to many cells.It is a good vector in the tissue-engineering field.However,its effect on osteoblasts remains unclear.OBJECTIVE：To study the cytotoxicity of chitosan thermosensitive hydrogel to osteoblasts.METHODS：Osteoblasts were cultured in chitosan thermosensitive hydrogel,and then viewed cell morphology under a microscope;at the same time,osteoblasts were cultured with different concentrations of chitosan thermosensitive extracts in vitro for 24,48,72,and 96 hours,respectively.The MTT assay was used to determine the relative growth rate（RGR） of osteoblasts and determined the level of cytotoxicity.RESULTS AND CONCLUSION：Osteoblasts were round at 24 hours after culture in chitosan thermosensitive hydrogel under a microscope,which extruded its horns and amplified after 48 hours.The RGF of osteoblasts cultured in different concentrations of chitosan thermosensitive extracts in vitro in each group was 92% to 112% at different time points,and the cytotoxic of all kind of concentrations of chitosan thermosensitive extracts were 0 or 1,which completely meet the standards of safety evaluation of biological materials.