改性纳米羟基磷灰石/PLGA材料同骨髓基质干细胞复合后相关生物学评价

Relative biological evaluation of hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly(lactide-co-glycolde) cultured with bone marrow mesenchymal stem cells

背景:改性纳米羟基磷灰石/聚乙交酯-丙交酯复合材料(L-lactic acid oligomer/Poly(lactide-co-glycolde),PLGA/g-HA)因具有良好的稳定性及机械性能,目前备受关注。目的:观察骨髓基质干细胞和改性PLGA/g-HA体外复合后的细胞活性及生物相容性。方法:原代培养兔骨髓基质干细胞,传代培养至第3代,MTT比色法检测在不同浓度PLGA/g-HA浸提液(10%、30%、50%、80%)中骨髓基质干细胞的增殖情况,以及骨髓基质干细胞在PLGA/g-HA表面的黏附性及其细胞形态。结果与结论:于培养后1,3d测得在不同浓度浸提液下骨髓基质干细胞的A值,10%浸提液组和对照组相比无显著性差异,4种浓度浸提液的细胞毒性均为1级;扫描电镜观察到骨髓基质干细胞在PLGA/g-HA表面逐渐伸展,形成伪足,最终牢固锚定在材料表面。提示在PLGA/g-HA的浸提液中骨髓基质干细胞能够发生增殖,对细胞无毒性。骨髓基质干细胞可以黏附在PLGA/g-HA表面且形态正常,生长状态良好,证明PLGA/g-HA具有良好的相容性和黏附性,可作为修复骨缺损的复合组织工程骨。

BACKGROUND：The hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly（lactide-co-glycolde）（PLGA/g-HA） composite material is preferable to good stability and favorable mechanical property.As scaffold cultured with bone marrow mesenchymal stem cells（BMSCs）.OBJECTIVE：To study the bioactivity and biocompatibility of BMSCs cultured with PLGA/g-HA composite material in vitro.METHODS：Third passage BMSCs of rabbits were cultured in PLGA/g-HA extract at different concentrations（10%,30%,50% and 80%）,and cell proliferation was detected by MTT method.Meanwhile,the cell adhesion and morphology were observed with scanning electron microscopy.RESULTS AND CONCLUSION：There was no significant difference between 10% group and negative group at 1 and 3 days after culture.The cytotoxic grade was Grade 1 in all the four concentrations.BMSCs could stretch pseudopodium gradually and adherent on the surface of PLGA/g-HA firmly.The results indicated that：①BMSCs could proliferate in PLGA/g-HA leaching liquor.②BMSCs have a good state on the surface of PLGA/g-HA.And we can apply the scaffold to bone defect repair,because of its good biocompatibility and cellular adhesion.