聚甲基丙烯酸甲酯颗粒对人滑膜细胞核因子κB受体激动剂配体/骨保护蛋白表达的影响

Effects of polymethyl methacrylate particles on human synoviocytes receptor activator of nuclear factor kappa B ligand /osteoprotegerin expression

背景:多种因子可以影响骨保护蛋白/核因子κB受体激动剂配体/核因子κB受体激动剂(Osteoprotegerin/receptor activator of nuclear factor-kappaB/ligand of receptor-activator of nuclear factor-kappaB,OPG/RANKL/RANK)系统的表达影响骨代谢,那么松动假体周围的骨水泥颗粒是否也通过影响其代谢参与假体松动过程?目的:观察聚甲基丙烯酸甲酯颗粒对人滑膜细胞RANKL/OPG表达的影响。方法:体外培养人滑膜细胞,在滑膜细胞培养体系中分别加入质量浓度为0(空白对照),0.2,1,10g/L的聚甲基丙烯酸甲酯骨水泥颗粒,采用荧光定量PCR检测上述各浓度PMMA颗粒作用不同时间后滑膜细胞内RANKL、OPG的表达量及其比值。结果与结论:与空白对照组相比,各实验组滑膜细胞内RANKL、OPG的表达量均有下降;随着与聚甲基丙烯酸甲酯颗粒共培养时间延长,各实验组滑膜细胞内RANKL、OPG表达均有下降趋势,而RANKL/OPG表达比值无明显变化。说明聚甲基丙烯酸甲酯颗粒在对滑膜细胞产生生物学反应时并不干扰假体周围骨代谢的动态平衡,并未直接参与人工关节假体的无菌性松动。

BACKGROUND：Various factors can influence bone metabolism through expression of osteoprotegerin/receptor activator of nuclear factor-κB/ligand of receptor-activator of nuclear factor-κB（OPG/RANKL/RANK） system.Whether bone cement particles also affects bone metabolic balance around prosthesis via OPG/RANKL/RANK expression in aseptic loosening remains poorly understood.OBJECTIVE：To investigate the effects of polymethyl methacrylate（PMMA） particles on the expression of RANKL/OPG in human synoviocytes.METHODS：In vitro,human synovial cells were cultured with different concentrations of PMMA particles（0,0.2,1 and 10 g/L）.The expression of RANKL,OPG and their ratio of each group was detected by fluorescence quantitative PCR at different time points.RESULTS AND CONCLUSION：Compared with the control group,the expression of RANKL and OPG in each experimental group was significantly down-regulated with time prolonged.While,there was no significant difference in the ratios of RANKL/OPG expression.Synoviocytes biologically reacting to PMMA particles had no interference on the dynamic balance of bone metabolism around prosthesis,thus play no obvious role in the aseptic loosening of artificial prosthesis.