用荧光检测方法研究氦-氖激光对小鼠腹腔巨噬细胞的活化作用

THE ACTIVATING ACTION OF He-Ne LASER IRRADIATION ON PERITONEAL MACROPHAGES IN MICE BY FLUORESCENT EXAMINATION METHOD

目的：探讨氦－ 氖（Ｈｅ－ Ｎｅ） 激光对机体的免疫刺激作用的机理。方法：用剂量为６３－７Ｊ／ｃｍ２ 的Ｈｅ－Ｎｅ 激光照射小鼠的神阙穴，时间分别为３ ，５ ，７ 和１０ ｄ ，每天照射５ ｍｉｎ，然后分别做如下实验：ａ．用荧光显微技术观察腹腔巨噬细胞（ Ｍψ） 对白色念珠菌的吞噬功能；ｂ ．α－ 萘酚醋酸酯法显示腹腔Ｍψ内非特异性酯酶（ＮＳＥ），并用图象分析系统对ＮＳＥ作定量分析。结果：各照射组吞噬率、吞噬指数及ＮＳＥ 的各项参数值均较对照组有非常显著性差异（ Ｐ＜ ０－０１） ，随照射天数增加，各项指标也随之增加，各照射组间比较也均有非常显著性差异（ Ｐ＜０－０１）。结论：适当剂量的Ｈｅ－ Ｎｅ 激光能活化Ｍψ，提高机体免疫功能。

Objective:To research He-Ne laser stimulating immunity of organism. Methods:Shenque acupoint of mice abdomen was irradiated by He-Ne laser at the dosages of 63.7J/cm 2 for 3,5,7 and 10 days respectively, 5 minutes each day. Then following methods are taken:a.Observing the phagocytosis to C. albicans of mice peritoneal macrophages (PMψ) with the fluorescence microscope after acridine orange (AO) staining. b.Measuring the non-specific esterase(NSE) in PMψ after being stained with α-naphthyl acetate by image analysis. Results:The above indices of irradiated groups were higher than that of non-irradiated group (P<0 01). Comparison between different irradiated groups also showed significant difference(P<0 01). Conclusion:It is suggested that the He-Ne laser with appropriated dosages can activate immunocytes, and enhance the immunity of organism.