激光照射对C57黑鼠膝骨关节炎软骨及热休克蛋白70的影响(英文)

Effects of laser irradiation on arthritic histopathology and heat shock protein 70 expression in C57 black mice with osteoarthritis

目的:观察不同波长激光照射C57黑鼠的犊鼻穴对骨关节炎模型关节软骨损伤的作用及对热休克蛋白70表达的影响。方法:C57黑鼠共60只分为6组,每组10只。其中1组为正常对照组,其余5组接受负荷训练。在电动跑台上进行1周的适应性训练后,以5m/min的速度连续训练50m,并人工驱赶保持其运动状态,每日1次,持续进行6周,以引发小鼠骨关节炎。造模成功后,除模型对照组和假治疗组外,其余3组每天分别接受10.6μm激光、650nm激光及二者的复合激光分别照射犊鼻穴,Mankin评分评定小鼠的软骨退化情况,免疫组织化学荧光染色检测热休克蛋白70在小鼠关节软骨的表达情况。结果:模型对照组及假治疗组的关节软骨Mankin评分高于正常对照组(P<0.01),复合激光组的关节软骨Mankin评分明显低于模型对照组(P<0.01)。与正常组比较,复合激光组、10.6μm激光组、650nm激光组、假对照组及模型对照组关节软骨的热休克蛋白70阳性表达均有增强,但复合激光组的热休克蛋白70阳性表达增强较其他组更为明显。结论:650nm及10.6μm和二者的复合激光针灸对膝关节软骨退变均有明显治疗效应,复合激光较单一激光更为明显。复合激光对关节软骨中热休克蛋白70阳性表达的增强亦较650nm及10.6μm单一激光明显。激光针灸对骨关节炎的治疗效应可能与热休克蛋白70的表达有关。10.6μm与650nm激光具有协同作用。

Objective：To study the effects of three different laser treatments（650 nm alone,10.6 μm alone and combined laser of 650 nm and 10.6 μm）on experimental osteoarthritis of the knees in C57 black mice.Methods：Sixty C57 black mice were divided randomly into 6 groups.Ten mice were assigned to a normal control group（no intervention）and the other 5 groups were subjected to a forced running regimen to induce osteoarthritis.One group was set as the model control group.The other 4 groups were given 90 s of a 650 nm laser,90 s of a 10.6 μm laser,90 s of a combined laser,or a sham treatment on acupoint Dubi（ST35）of the rear left leg 3 times per week for 4 weeks.The modified Mankin score was used to evaluate the degree of cartilage degradation.Immunohistochemical staining for heat shock protein（HSP）70 was conducted.Results：Mankin scores of the model control group and the sham control group were significantly higher than that of the normal control group（P0.01）.Mankin score of the combined laser group was significantly lower than that of the model control group（P0.01）.Compared with the normal control group,there was a significant induction of HSP70 in the arthritic chondrocytes of the combined laser group.Conclusion：The arthritic cartilage induced in C57 black mice improved significantly after combined laser treatment of 650 nm and 10.6 μm lasers.This effect may be related to the induction of HSP70 in the arthritic chondrocytes.The two different lasers appear to have a synergistic effect.