高迁移率族蛋白B1在香烟暴露大鼠模型中的表达

The significance of high mobility group box 1 protein in rat with chronic bronchitis induced by smoking

目的探讨高迁移率族蛋白B1(HMGB1)在香烟诱导的慢性支气管炎大鼠模型中的表达及作用。方法雄性SD大鼠随机分为正常对照组、吸烟模型组,每组14只。采用烟熏法建立慢性支气管炎大鼠模型。光镜下观察支气管肺组织病理形态学改变,分析支气管肺泡灌洗液(BALF)细胞计数和分类,用ELISA法检测大鼠血清中HMGB1的浓度,免疫组化法检测HMGB1在肺组织的表达。结果模型组大鼠肺组织的病理形态改变与人类慢性支气管的特点相一致。BALF中模型组细胞总数、单核巨噬细胞及中性粒细胞百分比与健康对照组比较,差异有显著性(P<0.05)。与对照组相比,模型组大鼠血清HMGB1的含量显著升高(P<0.05)。免疫组化染色可见HMGB1主要分布于支气管及肺泡上皮细胞,与对照组比较,模型组大鼠肺组织HMGB1的表达强度明显增强(P<0.05)。结论吸烟是慢性支气管炎的一个重要危险因素,HMGB1可能在慢性支气管炎的发病中起到一定的作用。

Objective To investigate the effects of high mobility group box 1（HMGB1） protein in rat with chronic bronchitis induced by smoking.Methods 28 male SD rats were randomly divided into control and smoking model groups（n=14）.Model of chronic bronchitis was established by exposure to cigarette smoke. The lung tissue section stained by HE was observed under optical microscope in order to study the alternation of histopathology.Total cell counts and classification in bronchoalveolar lavage fluid（BALF） analysis were performed.The concentration of HMGB1 in serum was detected by ELISA and the expression of HMGB1 in the lungs was detected by immunohistochemical method.Results Pathological changes of smoking model rat′ s lung tissue was consistent with that of chronic bronchitis.Compared with control group,there were significant difference in total cellular score,mononuclear macrophage and neutrophil percentage in BALF in model group（P〈0.05）.The concentration of HMGB1 in serum of model group was significantly increased compared with that of control group（P〈0.05）.HMGB1 was found in the bronchial and alveolar epithelial cells.Compared with the control group,the expression of HMGB1 in the lungs was obviously elevated in model group（P〈0.05）.Conclusions Smoking is a major risk factor of chronic bronchitis,HMGB1 may play a role in the pathogenesis of chronic bronchitis,but the exact mechanism needs further investigation.