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PTCH和SMO在大鼠肝癌中的表达及意义 被引量：1

Expression and significances of SMO and PTCH in hepatocellular carcinoma in rats
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摘要 目的探讨PTCH和SMO两种基因在大鼠肝癌中的表达及其意义。方法用二乙基亚硝胺诱导SD大鼠肝癌模型,实时荧光定量PCR法检测大鼠肝癌组织及相应癌旁良性组织中PTCH mRNA和SMO mRNA的表达。结果 13例肝癌癌组织中与其旁良性组织PTCH mRNA表达量为6.33±0.62与7.18±0.99,而SMO mRNA表达量为7.93±1.00与8.76±0.83,两组间均有统计学差异(P<0.05)。结论 PTCH和SMO是Sonic Hedgehog(SHH)信号通路激活的重要因子;SHH通路可能参与肝癌的发生。 Objective To investigate the gene expression and significances of PTCH and SMO in diethylnitrosamin（DEN）-induced primary hepatocellular carcinoma（HCC） in rats.Methods Rat HCC models were established by DEN-induction method.The gene expressions of PTCH and SMO in rat HCC tissues along with their adjacent benign tissues were determined by real time PCR.Results Of the 13 rats,the expression level of PTCH mRNA in HCC tissues and their benign adjacent tissues was 6.33±0.62 and 7.18±0.99（P0.05）,respectively.The expression level of SMO mRNA was 7.93±1.00 and 8.76±0.83（P0.05）,respectively.Conclusions PTCH and SMO are important regulation factors of the Sonic Hedgehog（SHH） signaling pathway.Thus,the SHH signaling pathway is probably involved in the tumorigenesis of HCC.
作者 颜朗 孔宪炳 朱艳志
机构地区 重庆医科大学附属第一医院肝胆外科
出处 《中国普通外科杂志》 CAS CSCD 北大核心 2011年第7期704-707,共4页 China Journal of General Surgery
基金 重庆市卫生局科研基金资助项目(2008-2-40)
关键词 肝肿瘤 二乙基亚硝胺 PTCH SMO 实时荧光定量PCR Liver Neoplasms Diethylnitrosamine PTCH Smo Real Time PCR
分类号 R735.7 [医药卫生—肿瘤]
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参考文献15

  • 1Evangelista M, Tian H, de Sauvage FJ. The hedgehog signaling pathway in cancer [ J ]. Cfin Cancer Res, 2006, 12(20 Pt 1 ) :5924 -5928.
  • 2Rubin LL, de Sauvage FJ. Targeling the Hedgebog pathway in cancer [J] . Nat Rev Drug Diseov, 2006, 5 ( 12 ) : 1026 - 1033.
  • 3Song Z, Yue W, Wei B, et al. Sonic hedgehog pathway is essential for maintenance of cancer stem-like cells in human gastric cancer[J]. PLoS One, 2011 , 6(3) :e17687.
  • 4Kelleher FC. Hedgehog signaling and therapeutics in pancreatic cancer[J]. Carcinogenesis, 2011 , 32 (4) :445 - 45l.
  • 5Chen G, Goto Y, Sakamoto R, et al. a crucial mediator of sonic hedgehog signaling in prostate cancer, functio-ns as a negative modulator for androgen receptor[J]. Bioehem Biophys Res Commun, 2011 , 404(3) :809 -815.
  • 6Cheng WT, Xu K, Tian DY, et al. Role of Hedgehog sig naling pathway in proliferation and invasiveness of hepatocel- lular carcinoma cells[J]. Int J Oncol, 2009, 34(3) : 829 - 836.
  • 7Chen XL, Cheng QY, She MR, et al. Expression of sonic hedgehog signaling components inhepatocellular carcinoma and cyclopamine-induced apoptosis through Bcl-2 downregulation in vitro[ J]. Arch Med Res, 2010, 41 (5) : 315 - 523.
  • 8张志敏,王阁,杨志祥,单锦露,陈川,许文,李琼,王东,李增鹏.间歇给药对DEN诱发大鼠肝癌模型的影响研究[J].重庆医学,2007,36(20):2035-2037. 被引量：3
  • 9Zhao Y, Tong C, Jiang I. Hedgehog regulates smoothenedactivity by inducing a conformational switch [ J ]. Nature, 2007, 450(7167) :252 -258.
  • 10Huang S, HE J, Zhang X, et al. Activation of the hedgehog pathway in human hepatocellular carcinomas [J]. CarcInogenesis ,2006,27 ( 7 ) : 1334 - 1340.

二级参考文献33

  • 1杨富春,郑树森,蒋天安.改良法大鼠原发性肝癌模型的建立[J].中华医学杂志,2004,84(23):2018-2019. 被引量：20
  • 2Johnson RL, Rothman AL, Xie J, et al. Human homolog of patched, a candidate gene for the basal cell nevus syndrome [ J ]. Science, 1996, 272 : 1668 - 1671.
  • 3Hahn H. Wicking C, Zaphiropoulous PG, et al. Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome[ J]. Cell, 1996, 85 : 841 -851.
  • 4Raffel C, Jenkins RB, Frederick L, et al. Sporadic medullobla- stomas contain PTCH mutations[J]. Cancer Res, 1997, 57: 842 - 845.
  • 5Reifenberger J, Wolter M, Weber RG, et al. Missense mutations in SMOH in sporadic basal cell carcinomas of the skin and primitive euroectodermal tumors of the central nervous system [ J ]. Cancer Res,1998, 58 : 1798 - 1803.
  • 6Yanai K, Nagai S Wada J. Hedgehog signaling pathway is a possible therapeutic target for gastric cancer [ J ]. J Surg Oncol, 2007, 95 : 55 - 62.
  • 7Berman DM, Karhadkar SS, Maitra A, et al. Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours[ J]. Nature, 2003, 425 : 846 - 851.
  • 8Dimmler A, Brabletz T, Hlubek F, et al. Transcription of sonic hedgehog, a potential factor for gastric morphogenesis and gastric mucosa maintenance, is up-regulated in acidic conditions [ J ].Lab Invest, 2003, 83:1829-1837.
  • 9Ramalho-Santos M, Melton DA, McMahon AP. Hedgehog signals regulate multiple aspects of gastrointestinal development [ J ]. Development, 2000, 127 : 2763 - 2772.
  • 10Ma Xiaoli, Chen Kai, Huang Shuhong, et al. Frequent activation of the hedgehog pathway in advanced gastric adenocarcinomas [ J]. Carcinogenesis, 2005, 26 : 1698 - 1705.

共引文献13

同被引文献12

  • 1Scales S J, de Sauvage FJ. Mechanisms of Hedgehog pathway activation in cancer and implications for therapy[J]. Trends Pharmacol Sci, 2009, 30(6):303-312.
  • 2Yang L, Xie G, Fan Q, et al. Activation of the hedgehog-signaling pathway in human cancer and the clinical implications[J]. Oncogene, 2010, 29(4):469-481.
  • 3Amakye D, Jagani Z, Dorsch M. Unraveling the therapeutic potential of the Hedgehog pathway in cancer[J]. Nat Med, 2013, 19(11):1410- 1422.
  • 4Huang S, He J, Zhang X, et al. Activation of the hedgehog pathwayin human hepatocellular carcinomas[J]. Carcinogenesis, 2006, 27(7):1334-1340.
  • 5Sicklick JK, Li YX, Jayaraman A, et al. Dysregulation of the Hedgehog pathway in human hepatocarcinogenesis[J]. Carcinogenesis, 2006, 27(4):748-757.
  • 6Chen JS, Huang XH, Wang Q, et al. Sonic hedgehog signaling pathway induces cell migration and invasion through focal adhesion kinase/AKT signaling-mediated activation of matrix metalloproteinase (MMP)-2 and MMP-9 in ]iver cancer[J]. Carcinogenesis, 2013, 34(1):10-19.
  • 7Incardona JP, Gaffield W, Kapur RP, et al. The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction[J]. Development, 1998, 125(18):3553-3562.
  • 8Xu FG, Ma QY, Wang Z. Blockade of hedgehog signaling pathway as a therapeutic strategy for pancreatic cancer[J]. Cancer Lett, 2009, 283(2): 119-124.
  • 9Ma H, Li HQ, Zhang X. Cyclopamine, a naturally occurring alkaloid, and its analogues may find wide applications in cancer therapy[J].Curr Top Med Chem, 2013, 13(17):2208-2215.
  • 10Wu XY, Che J, Sun KK, et al. Cyclopamine increases the radiosensitivity of human pancreatic cancer cells by regulating the DNA repair signal pathway through an epidermal growth factor receptor-dependent pathway[J]. Mol Med Rep, 2013, 8(4):979-983.

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中国普通外科杂志
2011年 第7期

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