β-catenin在L-02,WB,HepG2和SMCC-7721细胞中的表达及其意义

Expression and significance of β-catenin in the L-02,WB,HepG2 and SMCC-7721 cells

目的探讨β-catenin的异常表达在原发性肝癌的发生、分化中的意义。方法将4种细胞株进行贴壁培养传代。利用免疫细胞化学法检测β-catenin在人肝细胞株(L-02),人肝干细胞株(WB),肝癌细胞株(HepG2,SMCC-7721)中表达的特征。结果在L-02细胞株中,β-catenin几乎全部分布于细胞膜,而细胞质和细胞核几乎无表达;在WB细胞株中,β-catenin主要分布于细胞膜,细胞质和细胞核有较少表达;在HepG2和SMCC-7721细胞株中,β-catenin几乎全部分布于细胞质和细胞核,并且有较高表达,在SMCC-7721中的表达多于HepG2细胞株。结论β-catenin由细胞膜逐渐向细胞质和细胞核异位表达在原发性肝癌的发生、分化过程中可能具有重要作用。

Objective To investigate the abnormal expression of β-catenin and its role in the development and differentiation of primary hepatocellular carcinoma.Methods Four types of cell lines（L-02,WB,HepG2 and SMCC-7721） were attachment cultured and subcultured.The expression characteristics of β-catenin in those cells were detected by immunocytochemical staining.Results Of the L-02 cells,the β-catenin proteins were expressed totally on the cell membrane and none of them were expressed in the cytoplasm and nucleus.Of the WB cells,the β-catenin proteins were expressed mainly on the cell membrane and slightly expressed in the cytoplasm and nucleus.However,the β-catenin proteins were strongly and totally expressed in the cytoplasm and nucleus of HepG2 and SMCC-7721 cells,and the β-catenin expressions in SMCC-7721 cells were higher than those of the HepG2 cells.Conclusions The translocated expression of β-catenin from the cell membrane to cytoplasm and nucleus may play an important role in the development and differentiation of primary hepatocellular carcinoma.