CCl_4增敏的二乙基亚硝胺诱导大鼠原发性肝癌模型的建立

Establishment of diethylnitrosamin-induced rat model of primary hepatocellular carcinoma with sensitivity enhancement by CCl_4

目的探讨建立死亡率低、诱癌时间短、成功率高、操作方便及与人肝癌的发生过程相似的动物肝癌模型的方法。方法取8周龄Wistar大鼠40只,先给予50%CCl4油溶液按0.3 mL/100 g后肢皮下注射,每周2次,共2周;休息2周,然后给予95μg/mL的二乙基亚硝胺(DEN)自由饮水12周。结果整个诱癌过程大鼠无死亡,体质量增加,精神状态好;诱癌第16周所有大鼠诱发出肝细胞性肝癌。结论以CCl4增敏的DEN喂养可以方便、简单、稳定地建立大鼠肝癌模型。实验动物死亡率低,成模率高。

Objective To investigate a simple procedure to establish an ideal animal model of hepatocellular carcinoma（HCC） featuring low mortality,high success rate,short period for tumor induction and tumorigenesis profile similar to human HCC.Methods Forty Wistar rats,aged 2 months,were used.Firstly,all rats underwent subcutaneous hind-limb injection with 50% CCl4 oil solution at a dose of 0.3 mL/100 g twice a week for 2 weeks,then injection was discontinued for 2 weeks.After that,the rats were fed with diethylnitrosamin（DEN,95 μg/mL in drinking water） for 12 consecutive weeks.Results No death of rats occurred during the course of the study.The body weight of rats increased and the general state of rats was normal.HCC was successfully induced in all rats at the 16 weeks of the study.Conclusions A rat HCC model of low mortality and high success rate can be easily and stably induced by DEN feeding with sensitivity enhancement by CCl4.