创伤性全身炎症反应综合征大鼠动物模型的建立

Establishment of the rat model of traumatic systemic inflammatory response syndrome

目的探讨建立可重复的大鼠创伤性全身炎症反应综合征实验模型。方法 Wistar大鼠30只大鼠随机分为对照组(A组),脂多糖组(B组)和实验组(C组)。B组在完成股动脉、股静脉插管后注入脂多糖(LPS,12 mg/kg),C组采取手术切除1/3肝脏及锤击右侧股骨3次的方法,而A组在同等条件下不进行有创实验。2 h后采血测定血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、尿素氮(UN)和肌酐(Cr)的含量,12 h后处死大鼠,取大鼠肺及肝脏组织做病理切片并HE染色。结果 B组及C组大鼠在建模后血清AL T、AST、UN和Cr含量明显增高,与A组比较差异有统计学意义(P<0.01);C组大鼠血清UN,Cr含量较B组增高,差异有统计学意义(P<0.05)。光镜观察发现B组及C组大鼠的肝脏和肺脏有组织损伤及明显的炎性反应;B组及C组其肝脏及肺的病理变化差别不明显。结论此方法可较方便的制作大鼠创伤性全身炎症反应综合征动物模型。

Objective To establish a reproducible experimental rat model of traumatic systemic inflammatory response syndrome. Methods Thirty healthy Wistar rats were randomly assigned to the control group （group A）, the LPS injury group （group B） and the surgical treatment group （group C）. For preparing trauma model, the rats of group B were performed intravenous infusion of lipopolysaccharide （LPS, 12mg/kg）, and the rats of group C were cut the liver （1/3） and hammered right femur three times. 2 hours later after the treatment, the alanine aminot ransferase （ALT）, aspartete aminot ransferase （AST）, urea nit rogen （UN） and creatinine （Cr） in serum were surveyed. At the 12 hours, the rats were killed and the liver and lung were taken for HE staining and comparision. Nits The concentration of serous ALT, AST, UN and Cr in group B and C were significantly higher than that of group A （P〈0.01）. Compared to that of group B, Serous UN and Cr in group C were significantly higher （P〈0.05）. For inflammatory reaction and infiltration in liver and lung, there was no significant difference between group B and C. Conclusions The rat model of traumatic systemic inflammatory response syndrome can be established successfully using this surgical process.