钠/碘转运体在不同甲状腺组织中的表达

Expression of sodium/iodide symporter in different thyroid tissue

目的:检测钠/碘转运体(sodium/iodide symporter,NIS)在不同甲状腺组织中的表达以及在甲状腺乳头状癌中的分布。方法:选取正常甲状腺组织16例、结节性甲状腺肿组织14例、甲状腺乳头状癌23例,运用蛋白免疫印迹(Western blot)技术检测NIS的表达,并分开提取甲状腺乳头状癌胞浆、胞膜蛋白,分析NIS在甲状腺乳头状癌组织中的分布情况。结果:乳头状癌组、结节性甲状腺肿组NIS表达与正常组相比增高,差异有统计学意义(P<0.05);23例乳头状癌中,21例表达于胞浆,仅10例在胞膜上检测到,阳性率分别为91.3%和43.5%,两者差异有统计学意义(P<0.01);10例胞浆、胞膜均阳性者胞浆组NIS表达高于胞膜组,差异有统计学意义(P<0.01)。结论:在甲状腺乳头状癌、结节性甲状腺肿中NIS表达增高,但是在甲状腺乳头状癌中NIS主要表达在细胞浆中,而非细胞膜上。

Objective： To detect the expression of sodium/iodide symporter（NIS）in different thy-roid tissue,and the distribution of NIS in papillary thyroid carcinoma.Methods： sixteen cases of normal thyroid tissue,14 cases of nodular goiter and 23 cases of papillary thyroid carcinoma were selected,Western blot technique were used to detecte NIS expression in various tissues.Then,cytoplasm and membrane proteins of papillary thyroid carcinoma were extracted separately to analysis the distribution of NIS in papillary thyroid cancer.Results： The expression of NIS in PTC and NG was higher than that in NT.Difference between experimental groups was significant（P 0.05）;21 cases expressed in the cytoplasm in 23 cases of PTC,only 10 cases was detected in the membrane,the positive rate was 91.3% and 43.5%,the difference was statistically significant（P 0.01）.The expression of the NIS in cytoplasm was higher than that in membrane in 10 cases of positive in cytoplasm and membrane,the difference was statistically significant（P 0.01）.Conclusion： The NIS proteins in PTC and NG are over-expressed,but in PTC the NIS is mostly expressed in the cytoplasm rather than in the functional membrane.