3-甲基腺嘌呤对大鼠内皮祖细胞增殖、凋亡及细胞周期的影响

The effect of 3-MA on proliferation, apoptosis and cell cycle of rat endothelial progenitor cells

目的观察3-甲基腺嘌呤（3-methlyadenine，3-MA）对大鼠骨髓源性内皮祖细胞（endothelial progenitor cells，EPCs）增殖、凋亡及细胞周期的影响。方法密度梯度法取夫鼠骨髓EPCs，体外诱导分化并鉴定。实验分对照及不同浓度（1．25、2．5、5、10mmol／L）3-MA绀。四氮唑龄（methyl thiazolyl tetrazolium，M．rr）法测细胞增殖，流式细胞仪测凋亡率及细胞J吉J期。结果（1）5mmol／L组促EPCs增殖；10mmol／L组抑制EPCs增殖，差异有统计学意义（P〈0．05）。其余两组对EPCs增殖无明显影响，差异无统计学意义（P〉0．05）。（2）10mmol／L组促EPCs凋亡，差异有统计学意义（P〈0．05）。其余各组对EPCs凋亡率无明显影响，与对照组比较差异无统计学意义（P〉0．05）。（3）5、10mmol／L组影响细胞周期，5mmol／L组G0／G1期细胞减少、S和G2／M期增加；10mmol／L组发生S期阻滞，G，／M细胞减少；差异有统计学意义（P〈0．05）。结论3-MA对EPCs增殖及凋亡的影响与其浓度有关，当浓度为10mmol／L时抑制增殖并促进凋亡。

Objective Toinvestigate the effect of proliferation,apoptosis and cell cycle of 3-MA on rat endothelial progenitor cells. Methods Bone man＇ow-derived mononuclear cells were isolated from rat bone marrow by ficoll. There were five groups. The control group and four 3-MA concentration groups： 1.25 mmol/L, 2. 5 mmol/L,5 retool/L, 10 mmol/L. MTT was used to measure the proliferation of endothelial progenitor cells. Flow cytometry （FCM） was used to detect cell apoptosis and cell cycle. Results （ 1 ） 5 mmoL/L 3-MA promotes proliferation of endothelial progenitor cells, while 10 mmol/L 3-MA inhibits the proliferation of endothelial progenitor cells （P 〈 0. 05）. （2） 10 mmol/L 3-MA promotes apoptosis of endothelial progenitor cells, compared with the control, the difference was significant （P 〈 0. 05 ）. （3） 3-MA at the concentration of 5 mmoL/L reduces cells at G0/GI phase and increases S and G2/M phase cells; 10 mmol/L 3-MA induces endothelial progenitor cells blockade at S phase, G2/M phase cells decreased, compared with the control, the difference was significant （P 〈 0. 05）. Conclusions 5 mmo]/L 3-MA promotes the proliferation of endothelial progenitor cells. 10 mmol/l, 3-MA inhibits the proliferation and promotes apoptosis of endothelial progenitor cells.