纯钛表面含丹参钙磷涂层的制备及表征

Preparation and characteristics of biomimetic Ca-P coatings on titanium surfaces

目的研究在粗化纯钛表面采用仿生钙磷涂层法组装丹参的可行性。方法钛片表面粗化,采用仿生钙磷涂层技术,在纯钛表面建立不同浓度含丹参涂层,通过场发射扫描电子显微镜(FSEM)、傅立叶红外光谱(FTIR)、X射线衍射仪(XRD)和元素分析仪观察涂层晶体形貌、化学结构及组成,通过细胞培养验证其生物活性。结果 FSEM证实低浓度的丹参对钙磷涂层晶体的生长影响不大,高浓度的丹参对钙磷涂层晶体的生长影响较大;XRD和FTIR表明丹参并没有破坏涂层的化学结构和组成;元素分析结果表明实验组的碳元素质量比高于对照组;与对照组相比,实验组能够明显促进碱性磷酸梅(ALP)的表达。结论采用仿生钙磷涂层法可在粗化纯钛表面成功构建含丹参涂层。

Objective To investigate the feasibility of assembling biomimetic coatings including danshen on roughened titanium surfaces.Methods Surfaces of titanium plates were roughened.Then different danshen concentration coatings were deposited on titanium surfaces by biomimetic method.Field-emission scanning electron microscope（FSEM）,X-ray diffractometer（XRD） and fourier transform infrared spectroscopy（FT-IR） were applied to examine the morphology,structure and composition.Results FSEM indicated that low danshen concentration hardly affected the growth of Ca-P coating while the high concentration affected the growth.XRD and FTIR indicated that danshen did not change the chemical structure and composition of coatings.Elemental analysis showed Carbon in test group was higher than which in control group.Compared to the control group,test group showed increased ALP expression.Conclusion To apply the biomimetic methods can assemble the coating including danshen on roughened titanium surfaces.