期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

IGF-1对人牙周膜干细胞成骨能力的影响 被引量：3

Impact of IGF-1 on the osteogenic potential of human periodontal ligament stem cells
原文传递
导出
摘要 目的探讨IGF-1是否影响人牙周膜干细胞的成骨能力。方法分离、培养牙周膜干细胞,实验分为两组:对照组和实验组(经IGF-1诱导),进行矿化能力检测、实时定量RT-PCR检测、ALP活性检测。结果实验组人牙周膜干细胞体外矿化能力明显强于对照组。实验组人牙周膜干细胞Runx2,Collagen type I,Osteocalcin的表达以及ALP活性明显高于对照组。结论 IGF-1影响人牙周膜干细胞在体外的成骨能力。 Objective To investigate whether IGF-1 will impact the osteogenic potential of human periodontal ligament stem cells.Methods The periodontal ligament stem cells were isolated and cultured,and then divided into two groups： control group and experimental group（induced by IGF-1）.Mineralization detection,real-time quantitative RT-PCR detection,and ALP activity detection were all conducted.Results In the experimental group,the vitro mineralization of human periodontal ligament stem cells was stronger than that in the control group.Besides,the expression of human periodontal ligament stem cells such as Runx2,Collagen type I,and Osteocalcintron as well as the ALP activity in the experimental group was significantly higher than that in the control group.Conclusion IGF-1 impacts the osteogenic potential of human periodontal ligament stem cells in vitro.
作者 郑巍 王石
机构地区 南开大学附属口腔医院正畸科 天津市第三中心医院口腔科
出处 《现代口腔医学杂志》 CAS CSCD 2011年第4期286-289,共4页 Journal of Modern Stomatology
关键词 牙周膜干细胞 胰岛素样生长因子-1 Periodontal ligament stem cell Insulin-like growth factor-1
分类号 R780.2 [医药卫生—口腔医学]
  • 相关文献

参考文献11

  • 1陈芳,徐燕.牙周膜干细胞的研究进展[J].国际口腔医学杂志,2008,35(6):672-674. 被引量：4
  • 2Seo BM, Miura M, Gronthos S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet, 2004, 364(9429) : 149 - 155.
  • 3Molinelli A, Bonsignore A, Cicconi M, et al. IGF - 1 abuse in sport: clinical and medico - legal aspects. J Sports Med Phys Fitness, 2010, 50(4): 530-535.
  • 4Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulps tem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA, 2000, 97(25): 13625-13630.
  • 5Gronthos S, Brahim J , Li W, et al. Stem cell properties of human dental pulps tern cells. J Dent Res, 2002, 81 (8) : 531 -535.
  • 6Kurto~lu S, Kondolot M, Mazicio~lu MM, et al. Growth hormone, insulin like growth factor - 1, and insulin - like growth factor - binding protein- 3 levels in the neonatal period: a preliminary study. J Pediatr Endocrinol Metab, 2010, 23(9) : 885 -889.
  • 7Skamoto T, Nakagawa T, Horie RT. Inner ear drug delivery system from the clinical point of view. Acta Otolaryngol Suppl, 2010, 5 (563): 101 -104.
  • 8高秦,刘宏伟,金岩,聂鑫,刘源.人牙周膜干细胞的体外分离、纯化及初步鉴定[J].实用口腔医学杂志,2006,22(1):34-37. 被引量：34
  • 9Nixon AJ, Brower Toland BD, Sandell LJ. Primary nueleotide of predominant and alternate splice forms of equine insulin - like growth factor 1 and their gene expression patterns in tissues. Am J Vet Res, 1999, 6(5): 1234-1241.
  • 10Clemmons DR, Moses AC, Mckay MJ, et al. The combination of insulin - like growth factor - 1 dependent type diabetes : evidence for nviv obiological activity. Jclinen docrino lmetab, 2000, 8 (5): 1518 - 1524.

二级参考文献31

  • 1[1]Slack JM.Science,2000,287(5457):1431-1433.
  • 2[2]Weissnun IL Cell,2000,100(1):157-168.
  • 3[4]Seo BM,Miura M,Gronthos S,et al.Lancet,2004,364(9429):149-155.
  • 4[6]Techawattanswisal W,Nakahama K,Komaki M,et al.Biochem Biophys Res Commum,2007,357(4):917-923.
  • 5[7]Ivanovski S,Gronthos S,Shi S,et al.Oral Dis,2006,12(4):358-363.
  • 6[8]Dennis JE,Carbillet JP,Caplan AI,et al.Cells Tissues Organs,2002,170(2/3):73-82.
  • 7[10]Kawanabe N,Murakami K,Takano-Yamamoto T.Biochem Biophys Res Commun,2006,344(4):1278-1283.
  • 8[11]Gronthos S,Mankani M,Brahim J,et al.PNAS,2000,97 (25):13625-13630.
  • 9[12]Trubiani O,Di Primio R,Traini T,et al.Int J Immunopathol Pharmacol,2005,18(2):213-221.
  • 10[13]Sant'Ana AC,Marques MM,Barroso TE,et al.J Periodontol,2007,78(10):2007-2017.

共引文献35

同被引文献56

  • 1何晓宁,刘同军,石珊珊,程祥荣.壳聚糖胶原支架复合胰岛素样生长因子-1在牙周组织工程中的应用[J].武汉大学学报（医学版）,2006,27(3):323-325. 被引量：4
  • 2Molineli A,Bonsignore A,Cicconi M. IGF-1 abuse insport:clinical and medico-legal aspects[J].{H}Journal of Sports Medicine and Physical Fitness,2010,(04):530-535.
  • 3Kerstin S,Kristina A,Kamal M. Response of bone and periodontal ligament cells to biodegradable polymer scaffolds in vitro[J].{H}JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS,2010,(06):426-427.
  • 4Xu C,Lei C,Meng L. Chitosan as a barrier membrane material in periodontal tissue regeneration[J].J Biomed Mater Res B Appl Biomater,2012,(05):1435-1443.
  • 5Iwata T,Yamato M,Zhang Z. Validation of human periodontal ligament-derived cels as a reliable source for cytotherapeutic use[J].{H}Journal of Clinical Periodontology,2010,(12):1088-1099.
  • 6Choi MH,Noh WC,Park JW. Gene expression pattern during osteogenic differentiation of human periodontal ligament cels in vitro[J].J Periodontal Implant Sci,2011,(04):167-175.
  • 7Keogh MB,O' Brien FJ,Daly JS. A novel collagen scaffold supports human osteogenesis-applications for bone tissue engineering[J].{H}Cell and Tissue Research,2010,(01):169-177.
  • 8Horst OV,Chavez MG,Jheon AH. Stem cell and biomaterials research in dental tissue engineering and regeneration[J].{H}Dental Clinics of North America,2012,(03):495-520.
  • 9Costa EM,Silva S,Pina C. Evaluation and insights into chitosan antimicrobial activity against anaerobic oral pathogens[J].{H}ANAEROBE,2012,(03):305-309.
  • 10Tobita M,Mizuno H. Periodontal disease and periodontal tissue regeneration[J].{H}Current Stem Cell Research & Therapy,2010,(02):168-174.

引证文献3

二级引证文献4

现代口腔医学杂志
2011年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部