银杏叶提取物联合依达拉奉对气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO及NOS表达的影响

Effects of ginkgo biloba extraction combined with edaravone on expression of NO and NOS in Qi deficient and blood stasis type of rat brain tissue with cerebral ischemia reperfusion injury

目的:观察银杏叶提取物(ginkgo biloba extraction,EGb)与依达拉奉(edaravone,ED)联合应用对气虚血瘀型脑缺血再灌注损伤大鼠模型脑组织一氧化氮(NO)及一氧化氮合酶(NOS)表达的影响。方法:采用饥饿、疲劳、高脂饮食等复制大鼠气虚血瘀模型,再用线栓法阻断大脑中动脉2 h,再灌注治疗72 h后,观察EGb、ED及EGb+ED组对气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO及NOS表达的影响。结果:与模型组比较,EGb和ED均能降低气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO/诱导型NOS(iNOS)、总NOS(TNOS)含量,减轻神经细胞损伤(P<0.01),尤以两药联用效果更为显著(P<0.01)。结论:EGb联合EP能更好地降低气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO含量,其机制可能是通过降低脑组织iNOS、TNOS蛋白的表达而实现清除自由基,抗氧化,减轻神经细胞损伤,共同发挥脑保护作用。

Objective：To observe the effects of ginkgo blades extraction（EGb）combined with edaravone（ED）on expressions of NO and NOS in Qi deficient and blood stasis type of rat brain tissue with cerebral ischemia reperfusion injury.Methods：Rats model with Qi deficient and blood stasis were reproduced by hunger,fatigue and high-fat diet.Then the suture method was used to block up the middle cerebral artery for 2 hours,followed by 72 hours′ reperfusion therapy.The expressions of NO and NOS in brain tissue were evaluated on the rats model treated with EGb,ED and EGb combined with ED respectively.Results：Compared with model group,EGb and ED all decreased the levels of NO/iNOS and TNOS in Qi deficient and blood stasis type of rat brain tissues with cerebral ischemia reperfusion injury,and the injuries to neurons（P0.01）.The effect was significant especially in group of EGb combinied with ED（P0.01）.Conclusions：EGb associated ED can obviously inhibit the levels of NO in in Qi deficient and blood stasis type of rat brain tissues with cerebral ischemia reperfusion injury,and the mechanism of it may be through reducing the expression of iNOS and TNOS proteins in brain tissue to remove the free radicals,reduce nerve cell damages,and protect brain tissue.