p53、C-erbB-2癌基因蛋白在涎腺粘液表皮样癌中的表达及其临床病理学意义

Relationship between the expression or p53 and C-erhB-2 oncoproteins and biologic behaviors of human mucoepidermal carcinoma of salivary gland

目的探讨癌基因蛋白p53、C-erbB-2（p185）过度表达与粘液表皮样癌生物学行为的关系。方法利用微波免疫组织化学方法对32例人粘液表皮样癌的癌基因蛋白p53、C-erbB-2进行检测。结果正常诞腺组织p53、C-erbB-2均为阴性反应。癌旁导管上皮细胞两者阳性率分别为10.0％及15.0％，但腺泡细胞阴性。粘波表皮样癌组织中p53、C-erbB-2阳性率分别为40.6％及46.9％。p53、C-erbB-2在粘液细胞、表皮样细胞及中间细胞内均有表达。p53、C-erbB-2的表达与粘液表皮样癌肿瘤组织学类型、分化程度及肿瘤复发等肿瘤生物学行为密切相关（P＜0.01）。结论p53、C-erbB-2可作为粘液表皮样癌的分化性标志物，用于监测病情、判断预后等。

Objective To study the relationship between the expression of p53 and c-erbB-2 proteins and biologic behaviors of human mucoepidermal carcinoma. Methods The expressions of oncogene proteins p53 and c-erbB-2 in 32 cases of human mucoepidermal carcinomas were investigated with microwaveimmunohistochemistry. Results p53 and c-erbB-2 oncoproteins in duct epithelial cells around cancer tissues were 10. 0% and 15.0%,respectively, but those in the acinar cells affiliated with the salivary gland ducts were negative. The positive rates of p53 oncoprotein and c-erbB-2 oncoprotein in mucoepidermal carcinoma were 40. 6% and 46. 9%,respectively. Both p53 and c-erbB-2 oncoproteins were expressed in mucous cells,epidermoid cells and intermediate cells of mucoepidermal carcinoma. The expression of p53 and c-erbB-2 oncoproteins in human mucoepidermal carcinoma tissues were related to histological patterns,differentiation degree of cancer and tumor recurrence (P<0.01). Conclusion This study suggested that both p53 and c-erbB-2 oncoproteins,as the markers for differentiation of cancer,might be useful in screening progress and evaluating prognosis of patients with mucoepidermal carcinoma.