青春期病理性攻击动物模型的初步构建

Preliminary establish of the puberty pathological aggression animal model

目的构建理想的青春期病理性攻击动物模型。方法通过对青春期大鼠进行去奖赏性挫败实验与激惹刺激实验造模。采用旷野实验与糖水偏好实验、高架十字迷宫实验、嗅觉灵敏度实验评估其模型特异性。结果①病理性攻击组的总体攻击次数为[（101．17±2．85）次]，与普通攻击组[（52．5±5．36）次]、正常对照组[（8．83±1．34）次]比较均存在显著性差异（P〈0．01）；②各组大鼠各行为指标与总分呈中-高度正相关（r=0．379～0．929）；③病理性攻击组在危险部位攻击次数、屈服后继续攻击与攻击／威胁比与普通攻击组比较存在显著性差异（P〈0．01）；④病理性攻击组存在空间认知能力下降，但不表现抑郁、焦虑情绪和嗅觉障碍（P〉0．05）。而普通攻击组则存在明显抑郁情绪。结论各行为学指标满足青春期病理性攻击模型的评定标准，并且可以排除其他因素对模型特异性的干扰，提示该模型是较为理想的青春期病理性攻击模型。

Objective To establish the preferable puberty pathological aggression animal model. Methods The experimental models were established towards puberty rats by frustration test of non-reward and instigation test. Rat models were tested for specificity using open-field test, saccharine test, elevated plus-maze （EPM） and olfactory sensibility. Results （1）Compared with normal aggression（ 52.5 ± 5.36）and control group（ 8.83 ± 1.34） in total aggressive times, the pathological aggression group （101. 17 ± 2.85 ）increased significantly （P 〈 0.01 ） ;（2）Some behavior items of each model group were moderately or highly correlated to total score （ r = 0. 379 N 0. 929 ） ; （3） There was a significant difference between pathological aggression group and normal aggression group in the numbers of attack toward vulnerable-body regions, persistence attack after intruder displayed submissive and high attack/threat ratios （P〈0.01） ;（4）The pathological aggression group did not show depression,anxiety and olfactory disorder,except for space cognitive function（P 〉 0.05 ）. However,the normal aggression group displayed obvious depressive mood. Conclusion Each behavioral index matches the criteria of pathological aggression model. Meanwhile, it also excludes other factors of disturbing the specificity of the model. It suggests this model may be preferable puberty pathological aggression animal model.