Alzheimer病的T淋巴细胞功能活性检测

Immune-associated Function and Activity of T Lymphocyte in Alzheimer Disease

目的 探讨外周Ｔ淋巴细胞功能活性与Ａｌｚｈｅｉｍ ｅｒ病（ＡＤ）病情的关系。方法 流式细胞仪测定Ｔ细胞亚群；ＭＴＴ法检测ＣｏｎＡ刺激ＡＤ外周淋巴细胞的增殖功能；ＥＬＩＳＡ法检测细胞因子及其受体表达。结果 （１）ＣＤ４＋ 、ＣＤ８＋ Ｔ细胞比例和ＣＤ４＋ ／ＣＤ８＋ 比值各组间无统计学差异。（２）淋巴细胞增值在轻、重ＡＤ组略下降，但刺激指数（ＳＩ）都大于２，与对照无明显差异。（３）ＩＬ－２的分泌在重度ＡＤ组和ｓＩＬ－２Ｒ表达在轻、重度ＡＤ组明显升高（Ｐ＜ ０．０５），ＩＬ－２Ｒ的表达各组间差异无显著性。结论 Ｔ淋巴细胞亚群变化与ＡＤ病情的联系不明显，细胞因子分泌功能与ＡＤ进展有关。

Objective\ To investigate the relationship between the immune associated function and activity of T lymphocyte and severity of Alzheimer′s disease stage. Methods\ The patients were divided into two groups mild and moderately severe according to severity of disease stage, and their lymphocytes were compated to those of elderly controls. Monoclonal antibodies labeled by FITC were used to lymphocyte subset analysis. Lymphocytes were stimulation with ConA, we observed the proliferative ability, IL 2 secretion, cell membranal IL 2R expression and sIL 2R expression. Results\ 1. No significant differences were found in T lymphocyte subpopulations and the ratios of CD4/CD8 between groups (P>0 05). 2. All groups revealed strong response to ConA, there were no significant differences between AD and controls (P>0 05). 3. The IL 2 levels showed increase in AD groups and there was significant differences between moderately severe AD and controls (P<0 05).The amount of sIL 2R revealed an increase in AD groups, there were significant differences between AD and controls (P<0 05). The expression of IL 2R revealed no significant differences between AD and controls(P>0 05). Conclusions\ Our results indicated that immunologic parameters may be useful in monitoring progression of AD.