尿液PCA3基因检测在前列腺癌诊治中的临床意义

The siqnificance of urine PCA3 .qene in the dia clnosis and treatment of prostate cancer

目的探讨前列腺按摩后尿液中PCA3基因表达在前列腺癌诊断和治疗中的临床应用。方法收集前列腺增生患者34例，前列腺癌24例，取尿液，前列腺癌患者内分泌治疗后重新取尿液，分别离心后取沉淀物，用RT-PCR半定量方法检测PCA3表达情况。结果前列腺增生患者尿液中未找到PCA3基因，前列腺癌患者尿液PCA3基因表达呈阳性，内分泌治疗前后前列腺癌患者尿液PCA3基因表达有显著差异。结论尿液PCA3检测可以作为前列腺癌诊断的一个指标，可以对前列腺癌的治疗进行指导。它作为一种无创性诊断方法具有良好的前景。

Objective To investigate the expression ofPCA3 gene in urine of patients with prostate cancer after prostate massaging. Methods The expression of PCA3 gene was detected by RT-PCR in urine of 24 patients with prostate cancer, 34 patients with benigh prostate hyperplasia and 24 patients with prostate cancer after endocrine therapy. Results No expression of urine PCA3 gene was found in patients with benigh prostate hyperplasia, but positive expression in patients with prostate cancer. The urine PCA3 gene expression had significant difference before and after endocrine therapy. Conclusion Urine PCA3 gene can be used as an index for diagnosis and treatment of prostate cancer.