脑得生有效部位三七皂苷类成分含量测定

Determination of Panax notoginseng in Effective Composition of Naodesheng

目的:建立脑得生有效部位中三七皂苷类成分的含量测定方法。方法:采用高效液相色谱法,流动相为乙腈-水,梯度洗脱,流速为0.9 ml/min,检测波长为201nm;柱温为25℃。结果:人参皂苷Rg1的平均加样回收率为99.73%,RSD=1.59%,人参皂苷Rb1的平均加样回收率为99.16%,RSD=1.37%,三七皂苷R1的平均加样回收率为100.34%,RSD=1.90%。结论:该方法操作简便、易行、可用于脑得生有效部位中三七皂苷类成分的含量测定。

Objective：To establish a method for determing content of Panax notoginseng in effective parts of Naodesheng.Methods： Gradient elution with acetonitrile-water was used as the mobile phase.The flow rate was 0.9 ml/min,and the detection wavelength was set at 201 nm.Results： The average recoveries of ginsenoside Rg1 and Rb1 and notoginsenoside R1 were 99.73%（RSD=1.59%）,99.16%（RSD=1.37%）,100.34%（RSD=1.90%） respectively.Conclusions： The method is simple,accurate and can be used for quality control of the effective parts in Naodesheng.