摘要
为了探明紫玉兰花粉生活力测定方法以及适宜的贮藏方法,采用离体培养法、醋酸洋红染色法、碘-碘化钾染色法和TTC(氯化三苯基四氮唑)染色法等方法研究了花粉寿命长短与环境条件的关系。结果表明:4种测定方法中只有离体培养法适用于紫玉兰花粉生活力测定;培养基中添加不同浓度的蔗糖和硼酸对花粉的萌发有促进作用,紫玉兰花粉萌发的最适培养基为2%蔗糖+0.001%硼酸,萌发率达90.57%;在贮藏5 d的情况下,花粉最适宜的贮藏条件为-80℃,不干燥。
In order to explore appropriate storage method and optimum determination method and study the relationship between the pollen life and the storage environment,isolated culture,carmine acetate dyeing、I-KI staining and TTC staining methods were used to determine the fresh pollen ability of Magnolia liliiflora.The results showed that culture media with different concentration of sucrose and boric had good effects on the pollen germination.The pollen germinating percentage reached the highest（90.05%） after incubated on the media supplemented with 2% sucrose and 0.001% boric acid.The best conditions to keep vitality of pollen is in not dry environment at-80℃,the storage ability lasted for more than 5 d.
出处
《贵州农业科学》
CAS
北大核心
2011年第7期188-191,共4页
Guizhou Agricultural Sciences
基金
河南省创新人才工程项目(2005-126-49)
河南科技学院博士基金项目(050106)
河南科技学院重点基金项目(050121)
关键词
紫玉兰
花粉
培养基
染色
贮藏
Magnolia liliiflora
pollen
culture
dyeing
storage
