低分子RNA及5-Fu对白血病细胞系K562细胞抑制作用的实验观察

EFFECT OF LOW-MOLECULAR RNA AND 5-FU INHIBITING PROLIFERATION OF CELL IN LEUKEMIC K562 CELLS

目的：观察低分子ＲＮＡ 及５－ Ｆｕ 在白血病细胞系Ｋ５６２ 细胞中的作用。方法：在Ｋ５６２细胞培养中加低分子ＲＮＡ（ 实验组１） 、５ － Ｆｕ（ 实验组２） 、低分子ＲＮＡ 和５ － Ｆｕ（ 实验组３） 及Ｐ－Ｂ－Ｓ（ 对照组），观察集落细胞数，死、活细胞计数及细胞蛋白含量。结果：集落细胞数对照组为１４２ 个，实验组１ 为７０ 个，抑制率为５０－７ ％ ，实验组２ 为７１ 个，抑制率为５０－５％ ，实验组３ 为４１个，抑制率为７１－１ ％ ；活、死细胞实验组１ 为４２ 个、１１ 个，实验组２ 为４３ 、５ 个，实验组３ 为２７ 个、１６ 个；细胞蛋白含量实验组１ 为１－６１０ ，实验组２ 为１－６２２ ，实验组３ 为０－８７５ ，占７２－７ ％ 。结论：低分子ＲＮＡ能对白血病细胞系Ｋ５６２ 细胞的增殖有明显抑制作用，且能增强５ － Ｆｕ 抑制肿瘤细胞的增殖效果。

OBJECTIVE:To evaluate effect of low-molecular RNA and 5-Fu inhibiting cell proliferation of leukemic K562 cells. METHOD:Add low-molecular RNA(group one),5-Fu (group two),low-molecular RNA and 5-Fu (group three) and P.B.S (control group) to culture medium of K562 cells.Cell clusters live and dead cells were counted and cell protein was detected.RESULTS:Number of cell clusters:70 in group one,inhibiting rate 50.7%;71 in group two, inhibiting rate 50.5%;41 in group three,inhibiting rate 71.1%.Number of live and dead cells:42 and 11 in group one, 43 and 5 in group two, 27 and 16 in group three. 43 and 5 in group two,27 and 16 in group three.Cell protein:group one 1.610,group two 1.622 and group three 0.875, making up 72.7%.CONCLUSION:Low-molecular RNA can remarkably inhibit proliferation of leukemic K562 cells and strengthen the effect of 5-Fu inhibiting Proliferation of tumor cells.