皖南地区乙型肝炎病毒基因型和亚型的分布特征

Distribution of genotypes and subtypes of hepatitis B virus in the population of southern of Anhui Province

目的:研究皖南地区人群乙型肝炎病毒(hepatitis Bvirus,HBV)基因型和亚型流行及分布情况。方法:采用HBV基因型和亚型特异性引物PCR法,对皖南地区319例HBV DNA阳性患者进行基因型和亚型的检测。结果:皖南地区319例HBV感染者中,可检出基因型285例,检出率为89%,其中B型162例(57%),全部为Ba亚型;C型49例(17%),其中C1亚型38例,C2亚型9例,未分型2例;B+C混合型74例(26%)。B型感染者与C型感染者血清HBVDNA水平差异无统计学意义(P>0.05),但均高于B和C基因型混合感染患者(P<0.01)。结论:皖南地区人群HBV感染的基因型主要为B型、B+C混合型及C型;亚型则以Ba和C1亚型为主。

Objective：To investigate the distribution of genotypes and subtypes of hepatitis B virus（HBV） in the population living in southern area of Anhui Province.Methods： The genotypes were detected in 319 subjects infected with HBV by genotype/subtype-specific primer PCR.Results： HBV genotypes and subtypes were identified in 285 of the 319 serum samples（89%）,and genotype B,which totally fell into sub-genotype Ba,was seen in 162（57 %） and genotype C in 49（17%） which included 38 genotypes of C1,9 of C2 and failure of genotyping in 2.Mixed genotypes（B＋C） accounted for 26 %（74/285）.There was no significant difference between patients infected with genotype B and those with genotype C in terms of serum HBV DNA loads（P0.05）,and yet the loads were greater than that of mixed genotypes of B and C.Conclusion： Genotype B,mixed genotypes（B＋C） and genotype C are prevalent in the population living in southern of Anhui province,and subtypes of Ba and C1 are predominant.