一种新颖葡萄糖生化传感器的研制及其在鲁米诺电化学发光法测定葡萄糖含量的应用

Preparation of a Novel Biosensor for Glucose and Its Application to the Determination of Glucose by the Luminol-Electrochemiluminescence

将葡萄糖氧化酶1mg,纳米金及碳纳米管的复合材料2mg,无水乙醇1mL和10μL全氟磺酸用超声方法分散均匀后滴涂于玻碳电极表面,制成新颖的葡萄糖生化传感器(记作Nano-Au/CNT′s/GOD/GCE)。在含0.2mol·L^(-1)磷酸盐缓冲溶液及5.5×10^(-5)mol·L^(-1)鲁米诺存在的溶液中,用此生化传感器作工作电极,测得葡萄糖浓度在8.0×10^(-7)～5.0×10^(-4)mol·L^(-1)范围内与鲁米诺反应产生的化学发光强度呈线性关系,其检出限(3S/N)为8.0×10^(-8)mol·L^(-1)。同一支传感器连续5次测定,所得测定值的相对标准偏差为4.8%。将此传感器置于pH7.8磷酸盐缓冲溶液中,在4℃条件下保存2周后,在相同体系中进行测定时,其发光强度降低7.2%。

A mixture containing 1 mg of glucose oxidase（GOD）.2 mg of the composite of nano-gold and carbon nanotubes（Nano-Au/CNT＇s）,1 mL of absolute alcohol and 10μL of perfluorosulfonic acid,was dispersed ultrosonically and dropped onto the surface of glassy carbon electrode（GCE）,to prepare a novel biosensor （designated as Nano-Au/CNT＇s/GOD/GCE） for glucose.In a solution of 0.2 mol·L^（-1） PBS and in the presence of 5.5×10^（-5） mol·L^（-1） luminol,using the novel biasensor as working electrode,linear relationship between magnitude of CL of the CL reaction with luminol and values of concentration of glucose was obtained in the range of 8.0×10^（-7）-5.0×10^（-4）mol·L^（-1） with its detection limit（3S/N） of 8.0×10^（-8）mol·L^（-1）.Value of RSD（n=5） found with a same biosensor in successive determinations was 4.8%.The CL intensity was lowered to an extent of 7.2% when the biosensor was kept in the PBS of pH 7.8 at 4℃for 2 weeks.