银新杨组织培养快繁技术的研究

Research on rapid propagation technology by tissue culture of Populus alba×P. alba var. pyramidalis

以银新杨萌发芽为外置体进行组培快繁技术研究，结果表明，初代培养阶段适宜的培养基为附加6-BA0．1mg·L^-1的改良MS培养基；继代与增殖培养阶段适宜的培养基为附加6-BA0．1mg·L^-1以＋IAA0．5mg·L^-1的培养基，增殖倍率为12～18倍；生根培养阶段，以1／2MS＋NAA0．01mg·L^-1组合效果最好，15d根系基本形成。

The rapid propagation technology was studied with sprouts of Populus alba × P. alba var. pyramidalis as explant. The results showed that the 6 - BA 0.1 mg·L^-1 improved MS culture medium was the optimal medium for the primary culture stage, and the 6 - BA0.1mg·L^-1＋ IAA0.5 mg·L^-1 culture medium was the optimal medium for the subculture with 12 ~ 18 times of multiplication rate. The 1/2MS ＋ NAA0.01 mg·L^-1 was the best culture medium for the rooting period, which could form root system after 15 d.