Prox1基因在原发性肝细胞癌中的表达及其与病理学分级及临床分期的相关性研究

Study on Expression of Prox1 Gene and Relevance with Pathological Grade and Clinical Stage in Patients with Hepato cellular Carcinoma

目的检测Prox1(prospero-related homeobox 1)基因在原发性肝细胞癌(HCC)中的表达,探讨其与HCC病理学分级及临床分期的相关性。方法应用RT-PCR方法检测51例肝癌组织、癌旁组织和16例正常肝组织中Prox1基因表达,并分析其与HCC病理学分级和临床分期的相关性。结果 Prox1 mRNA在肝癌、癌旁及正常肝组织中均有表达,但肝癌组织(0.243±0.102)和癌旁组织(0.537±0.235)的表达量均明显低于正常肝组织(0.812±0.372),P<0.01或P<0.05,且肝癌组织中Prox1 mRNA表达量又明显低于癌旁组织(P<0.05)。肝癌组织中Prox1 mRNA的表达量在不同病理学分级(F=97.950,P<0.001)和临床分期(F=228.300,P<0.001)的肝癌组织中差异均有统计学意义,且各病理学分级和临床分期间两两比较,差异也均有统计学意义(P<0.001,P<0.01)。Prox1 mRNA表达与肝癌的病理学分级(r=-0.930,P<0.01)和临床分期(r=-0.980,P<0.01)呈负相关。结论 Prox1 mRNA表达可能与HCC的发生、发展有关,然而其是否为HCC的抑癌基因有待进一步证实。

Objective To detect the expression of Prox1（prospero-related homeobox 1） gene in primary hepatocellular carcinoma（HCC）,and to analyze the correlation of Prox1 gene expression with pathological grade and clinical stage of HCC.Methods The expressions of Prox1 gene in carcinoma tissues and adjacent cancerous tissues in HCC as well as normal liver tissues were detected by semi-quantitative RT-PCR,then the correlation of Prox1 gene expression with HCC pathological grade and clinical stage were analyzed.Results The expression of Prox1 gene in carcinoma tissues（0.243±0.102） and adjacent cancerous liver tissues（0.537±0.235） was significantly lower than that in normal liver tissue（0.812±0.372）,respectively（P〈0.01 or P〈0.05）.Furthermore,the expression of Prox1 gene in carcinoma tissues was significantly lower than that adjacent cancerous liver tissues（P〈0.05）.The expressions of Prox1 gene in different pathological grade（F=97.950,P〈0.001） and clinical stage were significantly different（F=228.300,P〈0.001）,and when compared with each other,the differences of pathological grade and clinical stage were also significant（P〈0.001 or P〈0.01）.The expressions of Prox1 gene in HCC carcinoma tissue were negatively correlated with pathological grade（r=-0.930,P〈0.01） and clinical stage（r=-0.980,P〈0.01） of HCC.Conclusions Expression of Prox1 gene may be related to the initiation and development of HCC,however,that whether Prox1 gene functions as tumor suppressor in HCC needs further investigation.