摘要
由荧光熄灭的 l 本氨基丙酸(LPA ) 的决心方法被开发。试金基于葫芦的联合[7 ] uril (CB [7 ]) 与巴马亭 hydrochloride (PAL ) 反应。面对 CB [7 ] ， PAL 的荧光被能被采用检测 LPA 的 LPA 熄灭。在最佳的条件下面，一个线性范围 3.63 ?? 噐??? 体 ???? 六 ??? ???????????
The determination method of L-phenylalartine （LPA） by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril （CB [7]） with palmatine hydrochloride （PAL） reaction. In the presence of CB [7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63 ×10^-8- 9.68 ×10^-6 mol/L and a detection limit 1.27 ×10^-8 mol/L of LPA were obtained. The relative standard deviation （R.S.D） was 1.8% obtained from a series of 11 standards each containing 6.05 ×10^-6 mol/L of LPA. This paper also discusses the mechanism of fluorescence indicator probe.
基金
supported by the Research Fund for the Doctoral Program of Higher Education of China(No. 20091404110001)
the foundation of Shanxi Normal University(No.ZR1007)
