免疫组化定量分析吸烟对大鼠胸主动脉中胱硫醚-γ-裂解酶的影响

Quantitative immunohistochemical analysis of the effect of smoking on the cystathionine-γ-lyase in the rat thoracic aorta

目的定量研究烟草烟雾对大鼠胸主动脉的胱硫醚-γ-裂解酶影响。方法 16只10周龄SD大鼠随机分为对照组(正常呼吸条件下饲养)和吸烟组(每天吸烟20支,连续90 d),每组8只。被动吸烟大鼠模型造完后断颈处死,取其近心端胸主动脉于10%的福尔马林固定1～2 d,采用免疫组化染色和显微图像定量分析法,观察大鼠胸主动脉胱硫醚-γ-裂解酶的面积密度和光密度。结果吸烟组的大鼠胸主动脉中胱硫醚-γ-裂解酶的表达面积密度明显低于对照组(6.79±1.04vs.14.98±2.32,P<0.01);其光密度值也明显低于对照组(0.15±0.04 vs.0.21±0.03,P<0.01)。结论吸烟可导致大鼠胸主动脉中的胱硫醚-γ-裂解酶含量降低。

Objective To quantitatively study the effect of tobacco smoking on cystathionine-γ-lyase in the rat thoracic aorta.Methods 16 10-week-old male SD rats were randomly divided into the control group（fed under normal breathing conditions） and smoking group（smoking 20 cigarettes per day,for 90 days,n = 8）.The rats were killed by cervical dislocation,and the thoracic aorta was taken and fixed in 10% formalin for 1 to 2 days.The sections were immunohistochemically stained for cystathionine-γ-lyase.The cystathionine-γ-lyase area density and optical density in the microscopic images of the rat thoracic aorta were quantitatively analyzed.Results The area density of cystathionine-γ-lyase expression in the thoracic aorta of the smoking group was 6.79 ± 1.04,significantly lower than that of 14.98±2.32 in the control group（P0.01）.The optical density in the smoking group was 0.15±0.04,also significantly lower than that of 0.21±0.03 in the control group（P0.01）.Conclusion Smoking can decrease the concentration of cystathionine-γ-lyase in the rat thoracic aorta.