急诊重症监护病房多药耐药鲍曼不动杆菌的分子流行病学调查

Homogeneous Analysis of Multidrug Resistant Acinetobacter baumannii in Emergency Intensive Care Unit

目的调查急诊重症监护病房(EICU)短期出现的多药耐药鲍曼不动杆菌的基因同源性,为医院感染的控制提供科学依据。方法 4株多药耐药鲍曼不动杆菌分离自EICU肺部感染患者痰标本,分离时间为2009年7月25日至8月7日。按《全国检验技术操作规程》要求操作,用全自动微生物分析仪进行细菌鉴定及药敏试验。用脉冲场凝胶电泳(PFGE)对4株鲍曼不动杆菌进行基因分型,明确其是否为同一菌株的克隆。结果 4株鲍曼不动杆菌对包括碳青霉烯类在内的多种抗生素耐药,经DNA分型分别为A型和B型,非同一克隆株。结论本组鲍曼不动杆菌为多药耐药菌株,不具同源性,可以排除相互传播的可能性。

Objective To investigate the drug resistance and homogeneous analysis of Acinetobacter baumanii in emergency intensive care unit（EICU）.Methods Four multidrug-resistant Acinetobacter baumannii（MDR-Ab） strains isolated from nosocomial inpatients from July 25 to September 7 in 2009 were collected and tested for drug sensitivity and MIC determination as well.The A.baumannii isolates were typed with pulsed-field gel electrophoresis（PFGE） to determine whether they derived from the same clone.Results Four isolates from nosocomial inpatients were resistant to multiple antibiotics including carbapenem.The PFGE types identified from four isolates were A and B.The A.baumannii isolates did not derived from the same clone.Conclusion The prevalence of nosocomial infection is not due to transmission of the same strains among different individuals in EICU.