VEGF-C和VEGF-D在肝内胆管癌组织中的表达与淋巴结转移的关系及临床意义

Expressions of VEGF-C and VEGF-D in Intrahepatic Cholangiocarcinoma and Its Relationship with Lymph Node Metastasis

目的:探讨胆管癌患者血管内皮生长因子C和D(vascular endothelial growth factor-C and-D,VEGF-C and VEGF-D)在胆管癌组织中的表达及其与肿瘤淋巴结转移的关系。方法:应用免疫组化SABC法及Real-time PCR法检测57例胆管癌组织和正常胆管组织中VEGF-C、VEGF-D蛋白及其mRNA的表达。结果:胆管癌组织VEGF-C和VEGF-D表达明显高于正常胆管组织(P<0.01),其中淋巴结转移组VEGF-C、VEGF-D的表达与淋巴结未转移组间统计学差异显著(P<0.05)。VEGF-C和VEGF-D在胆管癌组织中的表达与淋巴结转移有关(P<0.01)。结论:胆管癌细胞非摄入性高表达的VEGF-C和VEGF-D与淋巴结转移密切相关,可作为评估胆管癌患者预后的重要参考指标。

Objective： To explore the expression of vascular endothelial growth factor C and D in ICC and its relationship with tumor angiogenesis, lymph node metastasis. Methods： Immunohistochemistry（SABC） and Real-time PCR were used to detect the protein expression and mRNA level of VEGF-C and VEGF-D in 57 samples of ICC tissue and normal gastric tissue. Results： The positive expression rate of VEGF-C and VEGF-D in ICC tissue was significantly higher than in normal gastric tissues （P 〈0.01）. VEGF-C, VEGF-D expression in ICC group and lymph node metastasis group were significantly different （P 〈0.05）. VEGF-C and VEGF-D expression in Intrahepatic Cholangiocarcinoma were positively correlated with lymph node metastasis （P 〈0.01）. Conclusion： The un-intake high expression of VEGF-C and VEGF-D in ICC cells is closely related to lymph node metastasis. They can serve as the important reference indicator to assess theprognosis of ICC patients.