DNA提取方法对转基因甘蔗PCR检测的影响

Effects of DNA Extraction Methods on Transgenic Sugarcane PCR Detection

研究了不同提取方法和不同生长时期甘蔗提取的DNA对转基因甘蔗植株PCR检测的影响。结果表明,幼苗时期提取的甘蔗叶片模板DNA质量高于田间成熟植株;甘蔗幼苗时期的PCR检测易于成熟植株;CTAB法、SDS法和CTAB-SDS法提取的甘蔗叶片DNA均能满足幼苗期甘蔗抗性植株的PCR检测要求;对成熟转基因甘蔗植株而言,以CTAB-SDS法提取的DNA进行PCR检测的效果最佳。

The effects of three template DNA extraction methods from different growth stages on transgenic sugarcane PCR detection were compared. The results indicated that the quality of DNA extracting from seedling leaves was better than that of mature plants and mature stage, and PCR detections were easier; DNA extracted from sugarcane leaves with CTAB method, SDS method and CTAB-SDS method were all suitable for PCR detection of resistant plants in seedling stage. The quality of DNA extracted with CTAB-SDS method was the best for PCR detection of resistant plants in mature stage.