广藿香组织培养快繁技术的研究

Rapid Propagation of Pogostemon cablin via Tissue Culture

以广藿香幼嫩叶片为外植体,通过愈伤组织途经诱导出大量丛生芽,然后将丛生芽分别转接到含有不同质量浓度6-BA的增殖培养基和含有不同质量浓度IBA的生根培养基上,筛选出最适增殖培养基:MS+6-BA 0.1～0.2 mg.L-1,最适生根培养基:1/2 MS+IBA 0.25～1.0 mg.L-1。广藿香组培苗株高达5～6 cm时进行炼苗移栽,移栽成活率高达95%。

The young leaves of Pogostemon cablin were used as explants,and a large number of shoots were obtained through inducing callus.The shoots were transferred onto the multiplication media with different concentration of 6-BA,and onto the root-promoting media with different concentration of IBA.The optimum multiplication culture medium was obtained（MS ＋0.1 mg·L-1—0.2 mg·L-1 6-BA）,and the optimum root-promoting medium was obtained（1/2 MS ＋0.25 mg·L-1—1.0 mg·L-1 IBA）.When the height of the plantlets of Pogostemon cablin amounted to 5—6 cm,the survival percentage of plantlets Pogostemon cablin in the transplantation was 95%.