甘蓝型油菜硫甙含量对接种菌核病的反应(英文)

GLUCOSINOLATES RESPONSES OF OILSEED RAPE LINES TO INFECTION BY INOCULATING Sclerotinia sclerotiorum

研究了甘蓝型油菜硫苷含量与人工接种菌核病的互作关系。在油菜五叶期，人工接种菌核|病菌丝体，然后在接种当天和接种后第1、第3和第7d分析接种叶片和其它叶片的硫苷变化，所有参试［抗（耐）病和感病］品系都对人工接种菌核病菌丝体反应强烈，在接种第3叶，硫苷含量显著增加。但是抗（耐）病品系的硫苷增加不仅仅限于接种叶片，品系014硫苷增加在接种后第7d的非接种叶片最幼嫩的第7叶片最为明显。品系020硫苷增加在接种后第3d的非接种叶片最幼嫩的第5叶片最为显著。在所有硫苷组分中，吲哚簇硫苷增加最多。感病品种的硫苷变化不同于抗（耐）病品种，非接种叶片展示出流苷的明显下降，同样以吲哚簇硫苷在接种后第7d的最幼嫩第7叶片下降最为明显。结果表明：营养器官流苷含量及组份（吲哚簇硫苷）是油菜植物体内的自然抗性保护机制之一。

Ineraction between glucosinolate accumulation and disease resistance of oilseed rape (Brassica napus) were studied by inoculating with mycellial plugs S. sclerotiorum. When the third leaves were inoculated at the stage of five leaves of the lines, local and systemic changes in glucosinolate concentraions could be measured in 0, 1, 3 and 7 days after inoculation. All cultivars [resistant (tolerant) and susceptible]showed strong response to inoculation, and had a significant in-crease of gluasinolates in the third leaves of inoculation. However, the response of resistant cultivars was no restricted to inoc-ulated leaves. The increase of glucosinolates of uninoculated leaves in resistant line (014) was most conspicuous in youngest,seventh leaves, 7 days after inoculation. Anyway, in the resistant lines (020), the increase of glucosinolates of uninoculated leaves was most conspicuous in youngest, fifth leaves, 3 days after inoculation. Among glucosinolates, indolyl accumulated ma-inly. The response of susceptible cultivare was very different from resistan cultivare. Susceptible lines showed a clear decrease in glucosinolate conentrations in uninoculated leaves, specifically indolyl glucosinolate in seventh leaves, 7 days after inocula-tion. The result appeared tha glucosinolates of vegetative tissues is one of natural plant defence mechanisms against fungal dis-ease.