利用激光散射技术研究乙醇对人血红蛋白的影响

Research on the effect of ethanol on hemoglobin with laser scattering technique

研究了过量饮酒情况下乙醇对血液中血红蛋白(Hb)的粒度及其分子结构的影响,以浓度分别为200、300、400、500和600mg/dl的乙醇体外作用Hb 3h,采用动态光散射和激光拉曼散射光谱法对作用后的Hb的粒径和拉曼光谱的变化进行检测分析。结果发现,随着乙醇浓度增加,Hb分子粒径会稍减小,氧合Hb占总蛋白比例增加,Hb处于氧合紧密状态而不易释氧;但当乙醇浓度继续增加到600mg/dl时,Hb粒径开始变大,而氧合Hb占总蛋白比例减小。这些结果有助于解释酗酒者组织缺氧的有关机制。

To investigate the effect of ethanol in excessive drinking on hemoglobin, the size and size distribution,and the Raman spectra of hemoglobin in ethanol solutions with concentrations of 200 mg/all,300mg/dl, 400mg/dl, 500 mg/dl and 600mg/dl respectively were determined using dynamic light scattering and Raman spectroscopy. It is found that as the increase of ethanol concentration, the size of hemoglobin decreases, the number of oxygenated hemoglobin increase, but their ability of releasing oxygen decreases. When the ethanol concentration increases to 600 mg/dl, the size of hemoglobin begins to increase and the number of oxygenated hemoglobin decreases. These results would help to explain the mechanism of alcoholics＇ hypoxia.