气相色谱内标法测定黄芩提取物亚微乳中的乙醇残留

Determination of Residual Ethanol Solvents in Submicro-emulsion of the Extracts from Scutellaria by Internal Standard Method of GC

目的:建立黄芩提取物亚微乳中的乙醇残留量的测定方法。方法:毛细管气相色谱法,以甲醇为内标,色谱柱为SUPELCOWAX10聚乙二醇毛细管柱,检测器为氢焰离子化检测器;程序升温(初始温度80℃,以8℃.min-1速率升至120℃),载气为氮气,检测器为FID。结果:乙醇含量在0.101～1.005 g.L-1线性关系良好(R2=0.998 8);平均回收率100.42%,RSD2.27%;最低检测限3.03 mg.L-1。结论:该法灵敏、准确、可靠,适用于本品中乙醇残留量测定。

Objective：To determine the ethanol organic solvents in submicro-emulsion of the extracts from Scutellaria.Method：A simple gas chromatography（GC） method was developed with SUPELCOWAX10 polyethylene glycol capillary column with FID detector and methyl alcohol as the internal standard.The carrier gas was nitrogen.The initial column temperature was 80 ℃,then raised（8 ℃·min-1）to 120 ℃.Result：The linearity was good in the range of 0.101-1.005 g·L-1（R2=0.998 8）.The average recovery was 100.42%（RSD 2.27%）.The limit of detection was 3.03 mg·L-1.Conclusion：This GC method is proved to be accurate,sensitive and reliable,it can be used to determine the ethanol organic solvents in submicro-emulsion of the extracts from Scutellaria.