气相色谱法测定冠心苏合胶囊中土木香内酯和异土木香内酯的含量

Determination of Alantolactone and Isoalantoctone in Guanxinsuhe Capsules by Gas Chromatography

目的:建立冠心苏合胶囊中土木香内酯和异土木香内酯的含量的测定方法。方法:采用聚乙二醇20 000(PEG-20M)毛细管柱,FID检测器,程序升温:初始温度190℃,保持30 min,以120℃.min-1的速率升温至240℃,保持5 min;进样口温度260℃;检测器温度280℃。结果:在该色谱条件下,土木香内酯在48.96～244.8 mg.L-1呈良好的线性关系,异土木香内酯在51.28～256.4 mg.L-1呈良好的线性关系。结论:本方法灵敏、准确、重复性好,可用于冠心苏合胶囊的质量控制。

Objective：To establish a capillary GC method for the determination of alantoctone and isoalantoctone in Guanxinsuhe capsules.Method：The analytical column was PEG-20M capillary column with anFID detector.The column temperature was programmed from 190 ℃（holding 30 min） at a rate of 120 ℃·min^ -1 to 240 ℃（holding 5 min）.The injector temperature was 260 ℃.The detector temperature was set at 280 ℃.Result：The linear ranges of alantoctone were 48.96-244.8 mg·L^ -1,and the linear ranges of isoalantoctone were 51.28-256.4 mg·L ^-1.Conclution：The methed is sensitive,accurate and reliable,and can be used for quality control of Guanxinsuhe capsules.