五味子藤茎提取物急性毒性试验及对小鼠试验性肝损伤的保护作用

Acute Toxity and Protective Effects of Extracts from Caulis of Schisandra chinensis on Liver Injury in Mice

目的:观察五味子藤茎提取物的对小鼠急性毒性反应及其对CCl4致小鼠肝损伤动物模型的保护作用。方法:测定最大给药浓度(0.40 g.mL-1),最大体积(0.04 mL.g-1)的2次ig对小鼠最大耐受量(MTD),并采用CCl4致小鼠急性肝损伤模型,观察五味子藤茎提取物低、中、高剂量(3.03,4.55,9.09 g.kg-1)对肝损伤小鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)水平的影响。结果:五味子藤茎提取物对小鼠1次ig MTD为32 g.kg-1.d-1,五味子藤茎提取物各剂量组均能降低小鼠血清ALT,AST水平,与模型组比较,高、中剂量组差异显著(P<0.05,P<0.01)。结论:五味子藤茎提取物毒性较低,对CCl4致小鼠急性肝损伤具有较强的保护作用。

Objective：To evaluate the acute toxicity and the protective effects of the extraction from caulis of Schisandra chinensis on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Method：The maximum dosage（concentration 0.40 g·mL-1,dose 0.04 mL·g^-1） of the extraction from caulis of S.chinensis was used as ig administration in mice to measure the maximum tolerance dose（MTD）.With different doses of the extracts（ig 3.03,4.55,9.09 g·kg^-1）,the levels of ALT and AST in serum were observed for acute liver injury induced by CCl4 in mice.Result：The MTD of the extraction in mice was 32 g·kg^-1·d^-1.All dose groups of the extraction from caulis of S.chinensis could decrease the levels of ALT and AST in serum.Compared with the model control group,moderate and high dose groups could significantly decrease the levels of ALT and AST in serum（P〈0.05,P〈0.01）.Conclusion：The extraction from caulis of S.chinensin has low toxicity,and shows protective effect on acute liver injury induced by CCl4 in mice.