抗毒补心胶囊对环磷酰胺所致免疫抑制小鼠免疫功能的影响

Effects of Kangdu Buxin Capsule on Immune Function in Immuno-suppressed Mice Induced with Cyclophosphamide

目的:观察抗毒补心胶囊对环磷酰胺(CTX)所致免疫抑制小鼠免疫功能的影响。方法:注射CTX形成免疫低下小鼠模型,ig给予不同剂量的抗毒补心胶囊(4,8 g.kg-1)和阳性对照药香菇多糖片(10 g.kg-1),以小鼠腹腔巨噬细胞的吞噬率和吞噬指数,外周血白细胞数及淋巴细胞转化率为观察指标。结果:ig给予抗毒补心胶囊4 g.kg-1后小鼠腹腔巨噬细胞的吞噬率和淋巴细胞转化率分别为(63.2±10.9)%和(47.33±6.18)%,ig给予8 g.kg-1的为(57.4±10.2)%和(43.07±6.23)%。抗毒补心胶囊对CTX致小鼠外周血白细胞减少有明显提升作用(P<0.05),并能明显改善CTX所致小鼠腹腔巨噬细胞吞噬率和淋巴细胞转化率的降低(P<0.05)。结论:抗毒补心胶囊能增强CTX致免疫功能低下小鼠免疫功能,对免疫失衡机体具有保护作用。

Objective：To observe the effects of Kangdu Buxin Capsule on immune function in immuno-suppressed（IS）mice induced with cyclophosphamide（CTX）.Method：The IS model was produced by intraperitoneal injection（ip）of CTX with a dose of 50 mg·kg-1in mice.The mice were randomly divided into normal control group,immunity deficiency model group,Kangdu Buxin Capsule treated group and positive control group respectively.The changes in the number of WBC,the phagocytosis activity of macrophage,and the proliferation of T lymphocyte in the spleen of mice were observed.Result：Compared with model group,Kangdu Buxin Capsule could increase the amount of the peripheral blood WBC（P〈0.05）,the phagocytosis activity of macrophage,and the proliferation of T lymphocyte compared with the model group（P〈0.05）.Conclusion：Kangdu Buxin Capsule has the effect of improving immune in immuno-suppressed mice induced with CTX,and has protective effect on the immune function of mice.